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Titolo:
2 N-TERMINAL SELF-ASSOCIATION DOMAINS ARE REQUIRED FOR THE DOMINANT-NEGATIVE TRANSCRIPTIONAL ACTIVITY OF WT1 DENYS-DRASH MUTANT PROTEINS
Autore:
HOLMES G; BOTERASHVILI S; ENGLISH M; WAINWRIGHT B; LICHT J; LITTLE M;
Indirizzi:
UNIV QUEENSLAND,CTR CELLULAR & MOL BIOL ST LUCIA QLD 4072 AUSTRALIA UNIV QUEENSLAND,CTR CELLULAR & MOL BIOL ST LUCIA QLD 4072 AUSTRALIA UNIV QUEENSLAND,DEPT BIOCHEM ST LUCIA QLD 4072 AUSTRALIA MT SINAI SCH MED,BROOKDALE CTR MOL BIOL NEW YORK NY 00000
Titolo Testata:
Biochemical and biophysical research communications
fascicolo: 3, volume: 233, anno: 1997,
pagine: 723 - 728
SICI:
0006-291X(1997)233:3<723:2NSDAR>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR SUPPRESSOR GENE; WILMS-TUMOR; 2-HYBRID SYSTEM; IDENTIFICATION; CELLS; BCL-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
G. Holmes et al., "2 N-TERMINAL SELF-ASSOCIATION DOMAINS ARE REQUIRED FOR THE DOMINANT-NEGATIVE TRANSCRIPTIONAL ACTIVITY OF WT1 DENYS-DRASH MUTANT PROTEINS", Biochemical and biophysical research communications, 233(3), 1997, pp. 723-728

Abstract

Patients with Denys-Drash syndrome (DDS) have been shown to be constitutionally heterozygous for mutations of the WT1 gene. Almost all DDS mutations inactivate or remove the DNA-binding zinc finger region of WT1 and the resulting mutant proteins appear to act in a dominant negative manner. This may occur via WT1 self-association, which has been shown to involve the first 180 amino acids. By creating a series of N-terminal deletions, we have further investigated WT1 self-association using a yeast di-hybrid system and an in vitro protein binding assay. Our results suggest that there are two distinct domains within the N-terminal region facilitating self-association, residing from amino acids 1-45 and 157-253. Co-transfection of WT1 with progressively shorter N-terminal constructs demonstrates that both of these sites are requiredfor a dominant negative activity as assessed by activation of a reporter construct. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 21:50:11