Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Metal-induced expressing of mammal Metallothionein-1 gene in cyanobacteriato promote cadmium-binding preferences
Autore:
Guo, XX; Shi, DJ; Xu, XD; Ouyang, YS; Ru, BG;
Indirizzi:
Chinese Acad Sci, Inst Bot, Res Ctr Photosynth, Beijing 100093, Peoples R China Chinese Acad Sci Beijing Peoples R China 100093 100093, Peoples R China Peking Univ, Natl Lab Prot & Plant Genet Engn, Beijing 100871, Peoples R China Peking Univ Beijing Peoples R China 100871 ijing 100871, Peoples R China Michigan State Univ, US DOE, MSU, Plant Res Lab, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 s Lab, E Lansing, MI 48824 USA
Titolo Testata:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
fascicolo: 6, volume: 52, anno: 1999,
pagine: 806 - 810
SICI:
0175-7598(199911)52:6<806:MEOMMG>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROKARYOTIC METALLOTHIONEIN; LOCUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Shi, DJ Chinese Acad Sci, Inst Bot, Res Ctr Photosynth, Beijing 100093, Peoples R China Chinese Acad Sci Beijing Peoples R China 100093 Peoples R China
Citazione:
X.X. Guo et al., "Metal-induced expressing of mammal Metallothionein-1 gene in cyanobacteriato promote cadmium-binding preferences", APPL MICR B, 52(6), 1999, pp. 806-810

Abstract

The metal(zinc)-inducible smtA gene promoter (smt O-P) from cyanobacteria was applied for the expression of mouse MT-1 cDNA in the filamentous cyanobacterium Anabaena sp. PCC 7120 to enhance its metal-binding capability and to change its main binding specificity from zinc to cadmium. Shuttle expression vector pKT-MRE transformed the cyanobacterial cells by triparent conjugal transfer. Positive clones were screened and identified by streptomycin,DNA dot blot, SDS-PAGE and Western blot analysis. Photosynthetic oxygen evolution and metal atom absorption indicated that under the cadmium stress the metal-induced expression of foreign mMT-1 doubled their cadmium resistance and developed cells showing a much higher preference to absorb cadmium other than zinc in medium. The cadmium content in cell extract rose from 11%to 36%, and the cadmium cleared from media by transgenic cells rose from 18% to 62%. There was only a slight enhancement for zinc binding in the wildor transgenic type.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:21:52