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Titolo:
Separation of negatively charged isomeric quinones in acidic solution by capillary electrophoresis with reductive electrochemical detection
Autore:
Smith, AR; Kirchhoff, JR; Tillekeratne, LMV; Hudson, RA;
Indirizzi:
Univ Toledo, Coll Arts & Sci, Dept Chem, Toledo, OH 43606 USA Univ ToledoToledo OH USA 43606 ts & Sci, Dept Chem, Toledo, OH 43606 USA Univ Toledo, Coll Pharm, Dept Med & Biol Chem, Toledo, OH 43606 USA Univ Toledo Toledo OH USA 43606 ept Med & Biol Chem, Toledo, OH 43606 USA Univ Toledo, Coll Arts & Sci, Dept Biol, Toledo, OH 43606 USA Univ ToledoToledo OH USA 43606 ts & Sci, Dept Biol, Toledo, OH 43606 USA
Titolo Testata:
ANALYTICAL COMMUNICATIONS
fascicolo: 11-12, volume: 36, anno: 1999,
pagine: 371 - 374
SICI:
1359-7337(1999)36:11-12<371:SONCIQ>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME PYRROLOQUINOLINE QUINONE; ZONE-ELECTROPHORESIS; LIQUID-CHROMATOGRAPHY; FLAVIN REDUCTASE; BASIC DRUGS; PQQ; COFACTOR; DEHYDROGENASE; IDENTITY; ANALOGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Kirchhoff, JR Univ Toledo, Coll Arts & Sci, Dept Chem, 2801 W Bancroft St,Toledo, OH 43606 USA Univ Toledo 2801 W Bancroft St Toledo OH USA 43606 43606 USA
Citazione:
A.R. Smith et al., "Separation of negatively charged isomeric quinones in acidic solution by capillary electrophoresis with reductive electrochemical detection", ANAL COMMUN, 36(11-12), 1999, pp. 371-374

Abstract

A capillary electrophoresis method with reductive electrochemical detection was developed for the separation of the novel enzyme cofactor pyrroloquinoline quinone (PQQ) and three isomeric analogues. Tuning the reduction potential of the o-quinone moiety to a value more positive than the reduction potential for oxygen was accomplished by adjusting the capillary buffer to pH 2, thus eliminating the need for deoxygenation. To counter the suppression of the electroosmotic flow (EOF) at pH 2, a negative separation voltage of -22.5 V was applied to a 25 mu m id capillary resulting in migration of the anionic isomers toward the electrochemical detector. Fast and efficient separation was achieved in 0.15 mM phosphate buffer at pH 2. A mass detection limit for PQQ of 2 fmol was obtained with end-column detection. This approach may find utility for the separation and sensitive detection of a widerange of reducible quinones.

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Documento generato il 04/07/20 alle ore 03:28:26