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Titolo:
Stable expression of HIV-1 Nef induces changes in growth properties and activation state of human astrocytes
Autore:
Kohleisen, B; Shumay, E; Sutter, G; Foerster, R; Brack-Werner, R; Nuesse, M; Erfle, V;
Indirizzi:
GSF, Natl Res Ctr Hlth & Environm, Inst Mol Virol, Neuherberg, Germany GSF Neuherberg Germany & Environm, Inst Mol Virol, Neuherberg, Germany Bavarian Nord, D-82152 Martinsried, Germany Bavarian Nord Martinsried Germany D-82152 , D-82152 Martinsried, Germany Max Delbruck Ctr Mol Med, Berlin, Germany Max Delbruck Ctr Mol Med Berlin Germany ck Ctr Mol Med, Berlin, Germany GSF, Flow Cytometry Grp, D-85764 Munich, Germany GSF Munich Germany D-85764 , Flow Cytometry Grp, D-85764 Munich, Germany
Titolo Testata:
AIDS
fascicolo: 17, volume: 13, anno: 1999,
pagine: 2331 - 2341
SICI:
0269-9370(199912)13:17<2331:SEOHNI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; CENTRAL-NERVOUS-SYSTEM; NECROSIS-FACTOR-ALPHA; LONG TERMINAL REPEAT; CD4 DOWN-REGULATION; T-CELLS; DILEUCINE MOTIF; SURFACE CD4; IN-VITRO; REACTIVE ASTROCYTES;
Keywords:
Nef; HIV-1; astrocytes; neuropathogenesis; astrogliosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
69
Recensione:
Indirizzi per estratti:
Indirizzo: Kohleisen, B GSF, Res Ctr Hlth & Environm, Inst Mol Virol, Ingolstaedter Landstr 1, D-85764 Munich, Germany GSF Ingolstaedter Landstr 1 Munich Germany D-85764 , Germany
Citazione:
B. Kohleisen et al., "Stable expression of HIV-1 Nef induces changes in growth properties and activation state of human astrocytes", AIDS, 13(17), 1999, pp. 2331-2341

Abstract

Objective: Nef was shown to be the predominant viral protein expressed in HIV-1-infected astrocytes in vivo and in vitro suggesting a distinct role of Nef in this cell type. Nef-induced activation of T cells is well described, whereas the functional activities of Nef in astrocytes are unknown. Our aim was to examine the effect of Nef on growth properties and activation ofastrocytes. Design: Human Nef-expressing astrocytic cell lines were established by stable transfection With different wild-type and mutant nf genes derived from laboratory isolates and brain tissue. Methods: Nef-expressing astrocytes were characterized in terms of growth properties (proliferation, growth in soft agar, focus formation) and morphology. Apoptotic cell death and expression of activation markers were determined by fluorescent antibody cell sorting. Results: Astrocytic cell lines revealed persistent Nef expression - detectable at the levels of mRNA and protein - and showed altered growth propel-ties and morphology. Elevated expression of activation markers such as glialfibrillary acidic protein and CD88 (complement receptor C5a) was observed;these are regarded as markers for inflammatory processes in the brain. This effect was independent of the nef type or the expression level of the Nefprotein. In contrast with previous reports no evidence for increased apoptotic cell death was found in astrocytes expressing Nef stably. Conclusions: Our findings suggest that Nef changes the cellular propertiesof astrocytes, thus contributing to astrocyte activation and induction of astrogliosis in the central nervous system of individuals with AIDS. (C) 1999 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 20:13:48