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Titolo:
Frequency of CD8(+) T lymphocytes specific for lytic and latent antigens of Epstein-Barr virus in healthy virus carriers
Autore:
Benninger-Doring, G; Pepper, S; Deml, L; Modrow, S; Wolf, H; Jilg, W;
Indirizzi:
Univ Regensburg, Inst Med Mikrobiol & Hyg, D-93053 Regensburg, Germany Univ Regensburg Regensburg Germany D-93053 , D-93053 Regensburg, Germany Univ Mainz, Inst Virol, D-55131 Mainz, Germany Univ Mainz Mainz Germany D-55131 inz, Inst Virol, D-55131 Mainz, Germany
Titolo Testata:
VIROLOGY
fascicolo: 2, volume: 264, anno: 1999,
pagine: 289 - 297
SICI:
0042-6822(19991125)264:2<289:FOCTLS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LYMPHOBLASTOID CELL-LINES; GAMMA-ELISPOT-ASSAY; TRANSACTIVATOR PROTEIN; ADOPTIVE TRANSFER; PEPTIDE ANTIGENS; EBV INFECTION; RESPONSES; IMMUNITY; EPITOPE; CTL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Jilg, W Univ Regensburg, Inst Med Mikrobiol & Hyg, Franz Josef Strauss Allee 11, D-93053 Regensburg, Germany Univ Regensburg Franz Josef Strauss Allee 11 Regensburg Germany D-93053
Citazione:
G. Benninger-Doring et al., "Frequency of CD8(+) T lymphocytes specific for lytic and latent antigens of Epstein-Barr virus in healthy virus carriers", VIROLOGY, 264(2), 1999, pp. 289-297

Abstract

We investigated CD8(+) T cell frequencies of five different Epstein-Barr virus-specific cytotoxic T lymphocyte epitopes located within proteins of the replicative cycle and the latent state in healthy long-term Virus carriers with IFN-gamma enzyme-linked immunospot assay. Frequencies of the HLA-A3-restricted epitope RVRAYTYSK (RVR) whose minimal length was mapped in this study to amino acid position 148-156 of the immediate-early protein BRLF1 were compared with those of a further known HLA-A3-restricted epitope withinEBNA3A, RLRAEAQVK (RLR). Determination of frequencies of CD8(+) T lymphocytes directed against lytic antigen epitope RVR revealed that only one of eight donors recognized this epitope. Frequency was calculated to be 65 RVR-specific CD8(+) T lymphocytes per 10(6) PBMC. None of the HLA-A3-positive donors exhibited IFN-gamma release after antigenic stimulation with the EBNA3A-specific peptide epitope RLR. Furthermore, we chose three known HLA-B8-restricted epitopes, RAKFKQLL (RAK), FLRGRAYGL (FLR), and QAKWRLQTL (QAK), ofthe lytic protein BZLF1 and the latent protein EBNA3A. Examination of eight HLA-BB-positive virus carriers revealed that the BZLF1-specific epitope RAK was recognized by all donors with a median frequency of 233 RAK-specificCD8(+) T lymphocytes per 10(6) PBMC. Only 50% of these donors reacted against EBNA3A-specific epitope FLR and a minority (25%) reacted against EBNA3A-specific epitope QAK. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 01:30:59