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Titolo:
Quantitative determination of tolbutamide and its metabolites in human plasma and urine by high-performance liquid chromatography and UV detection
Autore:
Hansen, LL; Brosen, K;
Indirizzi:
Odense Univ, Inst Publ Hlth, DK-5000 Odense C, Denmark Odense Univ Odense Denmark C , Inst Publ Hlth, DK-5000 Odense C, Denmark
Titolo Testata:
THERAPEUTIC DRUG MONITORING
fascicolo: 6, volume: 21, anno: 1999,
pagine: 664 - 671
SICI:
0163-4356(199912)21:6<664:QDOTAI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT;
Keywords:
tolbutamide; 4-hydroxytolbutamide; carboxytolbutamide; high-performance liquid chromatography; ultraviolet detection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
5
Recensione:
Indirizzi per estratti:
Indirizzo: Hansen, LL Odense Univ, Inst Publ Hlth, Winslowparken 19,3rd Fl, DK-5000 Odense C, Denmark Odense Univ Winslowparken 19,3rd Fl Odense Denmark C , Denmark
Citazione:
L.L. Hansen e K. Brosen, "Quantitative determination of tolbutamide and its metabolites in human plasma and urine by high-performance liquid chromatography and UV detection", THER DRUG M, 21(6), 1999, pp. 664-671

Abstract

An isocratic, high-performance liquid chromatography method has been developed for simultaneous determination of the oral antidiabetic tolbutamide and two of its metabolites, 4-hydroxytolbutamide and carboxytolbutamide, in human plasma and urine. The method was based on simple one-step liquid-liquid extraction with tertiary-butyl methyl ether as extraction solvent. The chromatographic eluent was 23:77 (v/v) methanol: 0.01 M aqueous sodium acetate buffer pH 3.0, and the UV detection was performed at a wavelength of 230 nm. The limit of detection was 0.1 mu M for tolbutamide in plasma and 1.5 mu M, 0.5 mu M, and 0.75 mu M for carboxytolbutamide, 4-hydroxytolbutamide, and tolbutamide, respectively, in urine. The limit of quantitation was 0.5 mu M for tolbutamide in plasma and 2 mu M, 0.75 mu M, and 1.25 mu M for carboxytolbutamide, 4-hydroxytolbutamide, and tolbutamide, respectively, in urine. The overall mean recoveries ranged from 91% to 109% for tolbutamide inplasma and from 80% to 98% in urine for all three compounds.

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Documento generato il 19/01/20 alle ore 21:01:20