Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration
Autore:
Martinez, D; Tames, RS; Revilla, MA;
Indirizzi:
Univ Oviedo, Unidad Fisiol Vegetal, Dpto Biol Organismos & Sistemas, E-33005 Oviedo, Spain Univ Oviedo Oviedo Spain E-33005 ismos & Sistemas, E-33005 Oviedo, Spain
Titolo Testata:
PLANT CELL REPORTS
fascicolo: 1, volume: 19, anno: 1999,
pagine: 59 - 63
SICI:
0721-7714(199911)19:1<59:COIVSO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEHYDRATION; PLANTLETS; APICES; PEAR;
Keywords:
cryopreservation; germplasm conservation; hop; Humulus lupulus L.; shoot-tips;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Revilla, MA Univ Oviedo, Unidad Fisiol Vegetal, Dpto Biol Organismos & Sistemas, E-33005 Oviedo, Spain Univ Oviedo Oviedo Spain E-33005 emas, E-33005 Oviedo, Spain
Citazione:
D. Martinez et al., "Cryopreservation of in vitro-grown shoot-tips of hop (Humulus lupulus L.) using encapsulation/dehydration", PL CELL REP, 19(1), 1999, pp. 59-63

Abstract

A cryopreservation procedure using encapsulation/dehydration was established for I;hoot-tips obtained from in vitro-grown shoots of hop. After dissection, shoot-tips were encapsulated in medium with alginate and 0.5 M sucrose. Optimal conditions consisted of preculture for 2 days in solid medium with 0.75 M sucrose, or in increasing sucrose concentrations, desiccation for4 h with silicagel in a flow cabinet (16% water content) followed by rapidfreezing and slow thawing. Shoot recovery after freezing 60 min in liquid nitrogen was around 80%. No phenotypical changes were observed in the recovered plants from cryopreserved shoot-tips growing in the field.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 00:32:02