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Titolo:
TISSUE-SPECIFIC EXPRESSION OF ALPHA-MESSENGER AND BETA-MESSENGER RIBONUCLEIC-ACID ISOFORMS OF THE HUMAN MINERALOCORTICOID RECEPTOR IN NORMAL AND PATHOLOGICAL STATES
Autore:
ZENNARO MC; FARMAN N; BONVALET JP; LOMBES M;
Indirizzi:
UNIV PARIS 07,INST FEDERATIF RECH CELLULES EPITHELIALES,INSERM,U246 F-75870 PARIS 18 FRANCE UNIV PARIS 07,INST FEDERATIF RECH CELLULES EPITHELIALES,INSERM,U246 F-75870 PARIS 18 FRANCE UNIV ANCONA,OSPED REG TORRETTE,CLIN ENDOCRINOL I-60100 ANCONA ITALY
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 5, volume: 82, anno: 1997,
pagine: 1345 - 1352
SICI:
0021-972X(1997)82:5<1345:TEOAAB>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALDOSTERONE BINDING; RAT-BRAIN; PSEUDOHYPOALDOSTERONISM; CORTICOSTERONE; HYPERTENSION; PROMOTERS; KIDNEY; CELLS; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M.C. Zennaro et al., "TISSUE-SPECIFIC EXPRESSION OF ALPHA-MESSENGER AND BETA-MESSENGER RIBONUCLEIC-ACID ISOFORMS OF THE HUMAN MINERALOCORTICOID RECEPTOR IN NORMAL AND PATHOLOGICAL STATES", The Journal of clinical endocrinology and metabolism, 82(5), 1997, pp. 1345-1352

Abstract

Expression of the mineralocorticoid receptor (MR) is restricted to some sodium-transporting epithelia and a few nonepithelial target tissues. Determination of the genomic structure of the human MR (hMR) revealed two different untranslated exons (1 alpha and 1 beta), which splicealternatively into the common exon 2, giving rise to two hMR mRNA isoforms (hMR alpha and hMR beta). We have investigated expression of hMRtranscripts in renal, cardiac, skin, and colonic tissue samples by insitu hybridization with exon 1 alpha and 1 beta specific riboprobes, using an exon 2 probe as internal control. Specific signals for eitherexon 1 alpha- and 1 beta-containing mRNAs were detected in typically hMR-expressing cells in all tissues analyzed. hMR alpha and hMR beta were present in distal tubules of the kidney, in cardiomyocytes, in enterocytes of the colonic mucosa, and in keratinocytes and sweat glands. Interestingly, although both isoforms appear to be expressed at approximately the same level, the relative abundance of each message compared with that of exon a-containing mRNA strikingly differs among aldosterone target tissues, suggesting the possibility of other tissue-specific transcripts originating from alternative splicing. Finally, functional hypermineralocorticism was associated with reduced expression of hMR beta in sweat glands of two patients affected by Conn's and Liddle's syndrome, whereas normal levels of hMR isoforms were found in one case of pseudohypoaldosteronism. Altogether, our results indicate a differential, tissue-specific expression of hMR mRNA isoforms, hMR beta being down-regulated in situations of positive sodium balance, independently of aldosterone levels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:11:36