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Titolo:
The human REV1 gene codes for a DNA template-dependent dCMP transferase
Autore:
Lin, WS; Xin, H; Zhang, YB; Wu, XH; Yuan, FH; Wang, ZG;
Indirizzi:
Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 Ctr Toxicol, Lexington, KY 40536 USA
Titolo Testata:
NUCLEIC ACIDS RESEARCH
fascicolo: 22, volume: 27, anno: 1999,
pagine: 4468 - 4475
SICI:
0305-1048(19991115)27:22<4468:THRGCF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SACCHAROMYCES-CEREVISIAE GENE; UBIQUITIN-CONJUGATING ENZYME; ESCHERICHIA-COLI DINB; 2 HUMAN HOMOLOGS; INDUCED MUTAGENESIS; POLYMERASE-ZETA; YEAST RAD6; REPAIR; PROTEIN; DOMAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, ZG Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ol, Lexington, KY 40536 USA
Citazione:
W.S. Lin et al., "The human REV1 gene codes for a DNA template-dependent dCMP transferase", NUCL ACID R, 27(22), 1999, pp. 4468-4475

Abstract

DNA is frequently damaged by various physical and chemical agents. DNA damage can lead to mutations during replication. In the yeast Saccharomyces cerevisiae, the damage-induced mutagenesis pathway requires the Rev1 protein. We have isolated a human cDNA homologous to the yeast REV1 gene. The humanREV1 cDNA consists of 4255 bp and codes for a protein of 1251 amino acid residues with a calculated molecular weight of 138 248 Da. The human REV1 gene is localized between 2q11.1 and 2q11.2. We show that the human REV1 protein is a dCMP transferase that specifically inserts a dCMP residue oppositea DNA template G. In addition, the human REV1 transferase is able to efficiently and specifically insert a dCMP opposite a DNA template apurinic/apyrimidinic (AP) site or a uracil residue. These results suggest that the REV1transferase may play a critical role during mutagenic translesion DNA synthesis bypassing a template AP site in human cells. Consistent with its roleas a fundamental mutagenic protein, the REV1 gene is ubiquitously expressed in various human tissues.

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Documento generato il 02/07/20 alle ore 22:37:57