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Titolo:
The bovine mu-opioid receptor: cloning of cDNA and pharmacological characterization of the receptor expressed in mammalian cells
Autore:
Onoprishvili, I; Andria, ML; Vilim, FS; Hiller, JM; Simon, EJ;
Indirizzi:
NYU, Sch Med, Dept Pharmacol, New York, NY 10016 USA NYU New York NY USA 10016 Sch Med, Dept Pharmacol, New York, NY 10016 USA NYU, Sch Med, Dept Psychiat, New York, NY 10016 USA NYU New York NY USA 10016 Sch Med, Dept Psychiat, New York, NY 10016 USA CUNY Mt Sinai Sch Med, Dept Physiol & Biophys, New York, NY 10029 USA CUNYMt Sinai Sch Med New York NY USA 10029 ophys, New York, NY 10029 USA
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1-2, volume: 73, anno: 1999,
pagine: 129 - 137
SICI:
0169-328X(19991110)73:1-2<129:TBMRCO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
NALORPHINE-LIKE DRUGS; CHRONIC SPINAL DOG; MOLECULAR-CLONING; FUNCTIONAL EXPRESSION; OPIATE RECEPTOR; MESSENGER-RNA; MORPHINE-LIKE; RAT-BRAIN; BINDING;
Keywords:
cloning; mu-opioid receptor; bovine; gene expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Simon, EJ NYU, Sch Med, Dept Pharmacol, 550 1St Ave, New York, NY 10016 USA NYU 550 1St Ave New York NY USA 10016 ve, New York, NY 10016 USA
Citazione:
I. Onoprishvili et al., "The bovine mu-opioid receptor: cloning of cDNA and pharmacological characterization of the receptor expressed in mammalian cells", MOL BRAIN R, 73(1-2), 1999, pp. 129-137

Abstract

The cDNA coding for the bovine mu-opioid receptor has been cloned and sequenced. Conserved sequences from murine delta-receptor cDNA were used as primers in polymerase chain reaction (PCR) to amplify cDNA, prepared by reverse transcription of bovine brain mRNA. This cDNA was used to probe a bovine brain library. The partial sequence obtained was extended to provide the full length clone by PCR. The cDNA has an open reading frame of 1203 base pairs (bp) with a 3'-untranslated region of 1900 bp and a 5'-untranslated region of 265 bp. The protein contains 401 amino acids and has 94% amino acid identity with the human and 91% with the rat mu-opioid receptor. It has the putative seven transmembrane domains, characteristic of G protein-coupled receptors and contains 5 potential N-linked glycosylation sites near the N-terminus. Several potential phosphorylation sites and a putative palmitoylation site are also present. The receptor was stably expressed in HEK293 cells. The binding profile was found to be that of a typical mu receptor, i.e.,mu agonists and antagonists, but not delta and kappa ligands, bound with high affinity. Functional assays, namely, opioid stimulation of [S-35]GTP gamma S binding and inhibition of forskolin-activated adenylyl cyclase, were also found to be highly specific for mu-opioid agonists. The receptor was downregulated by chronic exposure to mu agonists but not delta or kappa agonists. Evidence is presented indicating that the cloned receptor is the sameas the bovine mu receptor previously purified to homogeneity in our laboratory. No evidence was found for genes for multiple mu-type opioid receptors. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:05:39