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Titolo:
Detection of placental growth hormone variant and chorionic somatomammotropin-L RNA expression in normal and diabetic pregnancy by reverse transcriptase-polymerase chain reaction
Autore:
Hu, LM; Lytras, A; Bock, ME; Yuen, CK; Dodd, JG; Cattini, PA;
Indirizzi:
Univ Manitoba, Dept Physiol, Winnipeg, MB R3E 3J7, Canada Univ Manitoba Winnipeg MB Canada R3E 3J7 ol, Winnipeg, MB R3E 3J7, Canada
Titolo Testata:
MOLECULAR AND CELLULAR ENDOCRINOLOGY
fascicolo: 1-2, volume: 157, anno: 1999,
pagine: 131 - 142
SICI:
0303-7207(19991125)157:1-2<131:DOPGHV>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBONUCLEIC-ACID EXPRESSION; HUMAN CYTOTROPHOBLASTS; ENDOVASCULAR INVASION; TERMINAL VILLI; MESSENGER-RNA; MELLITUS; LACTOGEN; INSULIN; WOMEN; GH;
Keywords:
gestational diabetes; human growth hormone and chorionic somatomammotropin gene family; PCR; placenta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Cattini, PA Univ Manitoba, Dept Physiol, 730 William Ave, Winnipeg, MB R3E3J7, Canada Univ Manitoba 730 William Ave Winnipeg MB Canada R3E 3J7 anada
Citazione:
L.M. Hu et al., "Detection of placental growth hormone variant and chorionic somatomammotropin-L RNA expression in normal and diabetic pregnancy by reverse transcriptase-polymerase chain reaction", MOL C ENDOC, 157(1-2), 1999, pp. 131-142

Abstract

Diabetes is a common complication encountered during pregnancy. Earlier studies indicated that diabetic placentas bear morphological alterations consistent with modified placental differentiation, including alterations in the villous cellular content, structure, and total surface. Limited data associating the diabetic status with the expression of terminal placental differentiation markers are available. The human growth hormone/chorionic somatomanamotropin (hGH/CS) family consists of five genes, one of which (GH-N) isexpressed efficiently in pituitary while the other four (CS-A, B, L, and hGH-V) are expressed in placenta and represent ultimate placental differentiation markers. We developed and applied a sensitive RT-PCR method coupled with diagnostic restriction digestion to determine the relative levels of the hGH/CS family in normal pregnancies and examine whether their mRNA expression pattern is altered in pregnancies complicated by diabetes. We show that relative hCS-L content changes during placental development. Specifically, normal term placentas express higher relative levels of hCS-L, lower relative hGH-V levels and a 70-fold lower hGH-V/CS-L mRNA ratio compared to early placentas. Also, many term placentas from diabetic pregnancies express lower relative levels of hCS-L mRNA and a much higher hGH-V/CS-L mRNA ratio compared to normal term placenta, resembling more an early placenta patternof expression. Thus, our study suggests that the expression of terminal placental differentiation markers, such as the hGH/CS genes, is altered in term placentas from these diabetics reflecting either impaired placental differentiation or post-differentiation impairment of normal placental function. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:23:58