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Titolo:
Gas chromatography-mass spectrometry assay for determination of ketamine in brain
Autore:
Blednov, YA; Simpson, VJ;
Indirizzi:
Univ Colorado, Hlth Sci Ctr, Dept Anesthesiol, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 Dept Anesthesiol, Denver, CO 80262 USA
Titolo Testata:
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
fascicolo: 2-3, volume: 41, anno: 1999,
pagine: 91 - 95
SICI:
1056-8719(199904/06)41:2-3<91:GCSAFD>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SHORT-SLEEP MICE; LONG-SLEEP; PLASMA; NORKETAMINE;
Keywords:
ketamine assay; pharmacokinetic/pharmacodynamic; gas chromatography/mass spectrometry; mouse brain assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Simpson, VJ Univ Colorado, Hlth Sci Ctr, Dept Anesthesiol, 4200 E 9th Ave,Denver, CO 80262 USA Univ Colorado 4200 E 9th Ave Denver CO USA 80262 CO 80262 USA
Citazione:
Y.A. Blednov e V.J. Simpson, "Gas chromatography-mass spectrometry assay for determination of ketamine in brain", J PHARM TOX, 41(2-3), 1999, pp. 91-95

Abstract

A sensitive and precise gas chromatography-mass spectrometry method with selected ion monitoring has been developed for determination of ketamine in the brain using chlorpheniramine as an internal standard. The assay is based on the acid extraction of brain homogenate with hexane and ethyl ether with subsequent alkaline ethyl ether extraction. The analytical procedure hasa coefficient of variation of 3.0-5.3% and from 3.8 to 6.1% for extractionfrom water or spiked brain samples, respectively. The lowest detectable level of ketamine was 1 ng in any brain region. This level of detection was used to measure the ketamine concentrations in cerebellum brain stem, midbrain, hypothalamus, and cortex of C57B1/6 mice at awakening following intraperitoneal injection of a hypnotic dose. The ketamine concentrations in mousebrain were in the range from 41.6 to 48.6 ng/mg of tissue. (C) 1999 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 01:40:51