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Titolo:
Ca2+/calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena
Autore:
Gonda, K; Katoh, M; Hanyu, K; Watanabe, Y; Numata, O;
Indirizzi:
Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan Univ TsukubaTsukuba Ibaraki Japan 3058572 sukuba, Ibaraki 3058572, Japan Jobu Univ, Gunma 3728588, Japan Jobu Univ Gunma Japan 3728588Jobu Univ, Gunma 3728588, Japan
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 21, volume: 112, anno: 1999,
pagine: 3619 - 3626
SICI:
0021-9533(199911)112:21<3619:CAPCRA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFFECTING CELL-DIVISION; ACTIN-BINDING PROTEIN; MUTANT-GENE PRODUCT; SEA-URCHIN EGGS; CLEAVAGE FURROW; ALPHA-ACTININ; LOCALIZATION; CALMODULIN; MYOSIN; CALCIUM;
Keywords:
p85; calmodulin; cytokinesis; division furrow; Tetrahymena; W7;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Numata, O Univ Tsukuba, Inst Biol Sci, Tsukuba, Ibaraki 3058572, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058572 araki 3058572, Japan
Citazione:
K. Gonda et al., "Ca2+/calmodulin and p85 cooperatively regulate an initiation of cytokinesis in Tetrahymena", J CELL SCI, 112(21), 1999, pp. 3619-3626

Abstract

Tetrahymena p85 differs in mobility in two-dimensional SDS-polyacrylamide gel electrophoresis between wild-type and temperature-sensitive cell-division-arrest mutant cdaA1 cell extracts, and is localized to the presumptive division plane before the formation of the division furrow The p85 containedthree identical sequences which show homology to the calmodulin binding site of Ca2+/calmodulin dependent protein kinase Type II in Saccharomyces cerevisiae. We found the p85 directly interacts with Tetrahymena calmodulin ina Ca2+-dependent manner, using a co-sedimentation assay. We next examined the localization of p85 and calmodulin during cytokinesis using indirect immunofluorescence. The results showed that both proteins colocalize in the division furrow. This is the first observation that calmodulin is localized in the division furrow. Moreover, the direct interaction between p85 and Ca2+/calmodulin was inhibited by Ca2+/calmodulin inhibitor N-(6-aminohexyl)5-chloro-1-naphthalenesulfonamide HCl. When the cells were treated with the drug just before the beginning of cytokinesis, the drug also inhibited the localization of p85 and calmodulin to the division plane, and the formation of the contractile ring and division furrow. Therefore, we propose that theCa2+/calmodulin signal and its target protein p85 cooperatively regulate an initiation of cytokinesis and may be also concerned with the progression of cytokinesis in Tetrahymena.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:38:19