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Titolo:
A nuclear factor, ASC-2, as a cancer-amplified transcriptional coactivatoressential for ligand-dependent transactivation by nuclear receptors in vivo
Autore:
Lee, SK; Anzick, SL; Choi, JE; Bubendorf, L; Guan, XY; Jung, YK; Kallioniemi, OP; Kononen, J; Trent, JM; Azorsa, D; Jhun, BH; Cheong, JH; Lee, YC; Meltzer, PS; Lee, JW;
Indirizzi:
NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA NHGRI Bethesda MD USA 20892 anc Genet Branch, NIH, Bethesda, MD 20892 USA Pusan Natl Univ, Coll Pharm, Pusan 609735, South Korea Pusan Natl Univ Pusan South Korea 609735 harm, Pusan 609735, South Korea Kwangju Inst Sci & Technol, Dept Life Sci, Kwangju 500303, South Korea Kwangju Inst Sci & Technol Kwangju South Korea 500303 00303, South Korea Chonnam Natl Univ, Ctr Ligand & Transcript, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea Chonnam Natl Univ, Hormone Res Ctr, Kwangju 500757, South Korea Chonnam Natl Univ Kwangju South Korea 500757 Kwangju 500757, South Korea
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 48, volume: 274, anno: 1999,
pagine: 34283 - 34293
SICI:
0021-9258(19991126)274:48<34283:ANFAAA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROID-HORMONE RECEPTOR; ACTIVATION FUNCTION AF-2; HUMAN BREAST-CANCER; ESTROGEN-RECEPTOR; HISTONE ACETYLTRANSFERASE; MEDIATED TRANSACTIVATIONS; CRYSTAL-STRUCTURE; BINDING DOMAIN; RETINOIC-ACID; CO-REPRESSOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
76
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, JW NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA NHGRI Bethesda MD USA 20892 t Branch, NIH, Bethesda, MD 20892 USA
Citazione:
S.K. Lee et al., "A nuclear factor, ASC-2, as a cancer-amplified transcriptional coactivatoressential for ligand-dependent transactivation by nuclear receptors in vivo", J BIOL CHEM, 274(48), 1999, pp. 34283-34293

Abstract

Many transcription coactivators interact with nuclear receptors in a ligand- and C-terminal transactivation function (AF2)-dependent manner. We isolated a nuclear factor (designated ASC-2) with such properties by using the ligand-binding domain of retinoid X receptor as a bait in a yeast two-hybridscreening. ASC-2 also interacted with other nuclear receptors, including retinoic acid receptor, thyroid hormone receptor, estrogen receptor alpha, and glucocorticoid receptor, basal factors TFIIA and TBP, and transcription integrators CBP/p300 and SRC-1. In transient cotransfections, ASC-2, eitheralone or in conjunction with CBP/p300 and SRC-1, stimulated ligand dependent transactivation by wild type nuclear receptors but not mutant receptors lacking the AF2 domain. Consistent with an idea that ASC-2 is essential forthe nuclear receptor function in vivo, microinjection of anti-ASC-2 antibody abrogated the ligand-dependent transactivation of retinoic acid receptor, and this repression was fully relieved by coinjection of ASC-2-expressionvector. Surprisingly, ASC-2 was identical to a gene previously identified during a search for genes amplified and overexpressed in breast and other human cancers. From these results, we concluded that ASC-2 is a bona fide transcription coactivator molecule of nuclear receptors, and its altered expression may contribute to the development of cancers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 15:05:22