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Titolo:
Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa
Autore:
Alvarez, AH; Moreno-Sanchez, R; Cervantes, C;
Indirizzi:
Univ Michoacana, Inst Invest Quim Biol, Morelia 58030, Michoacan, Mexico Univ Michoacana Morelia Michoacan Mexico 58030 a 58030, Michoacan, Mexico Inst Nacl Cardiol, Dept Bioquim, Mexico City 14080, DF, Mexico Inst Nacl Cardiol Mexico City DF Mexico 14080 xico City 14080, DF, Mexico
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 23, volume: 181, anno: 1999,
pagine: 7398 - 7400
SICI:
0021-9193(199912)181:23<7398:CEBMOT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMID-DETERMINED RESISTANCE; ALCALIGENES-EUTROPHUS; NUCLEOTIDE-SEQUENCE; EXPRESSION; FLUORESCENS; CLONING; ATPASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Cervantes, C Univ Michoacana, Inst Invest Quim Biol, Edificio B-3,Ciudad Univ, Morelia 58030, Michoacan, Mexico Univ Michoacana Edificio B-3,Ciudad Univ Morelia Michoacan Mexico 58030
Citazione:
A.H. Alvarez et al., "Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa", J BACT, 181(23), 1999, pp. 7398-7400

Abstract

Everted membrane vesicles of Pseudomonas aeruginosa PAO1 harboring plasmidpCRO616, expressing the ChrA chromate resistance protein, accumulated fourtimes more (CrO42-)-Cr-51 than vesicles from plasmidless cells, indicatingthat a chromate efflux system functions in the resistant strain. Chromate uptake showed saturation kinetics with an apparent K-m of 0.12 mM chromate and a V-max of 0.5 nmol of chromate/min per mg of protein. Uptake of chromate by vesicles was dependent on NADH oxidation and was abolished by energy inhibitors and by the chromate analog sulfate. The mechanism of resistance to chromate determined by ChrA appears to be based on the active efflux of chromate driven by the membrane potential.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 00:49:31