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Titolo:
Different integrins mediate cell spreading, haptotaxis and lateral migration of HaCaT keratinocytes on fibronectin
Autore:
Koivisto, L; Larjava, K; Hakkinen, L; Uitto, VJ; Heino, J; Larjava, H;
Indirizzi:
Univ British Columbia, Fac Dent, Vancouver, BC V6T 1Z3, Canada Univ British Columbia Vancouver BC Canada V6T 1Z3 ver, BC V6T 1Z3, Canada Univ Turku, Medic Res Lab, Turku, Finland Univ Turku Turku FinlandUniv Turku, Medic Res Lab, Turku, Finland Univ Jyvaskyla, Dept Biol & Environm Sci, Jyvaskyla, Finland Univ Jyvaskyla Jyvaskyla Finland iol & Environm Sci, Jyvaskyla, Finland
Titolo Testata:
CELL ADHESION AND COMMUNICATION
fascicolo: 3, volume: 7, anno: 1999,
pagine: 245 - 257
SICI:
1061-5385(1999)7:3<245:DIMCSH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; ALPHA(V) INTEGRINS; RECEPTOR FUNCTIONS; EPITHELIAL-CELLS; EXPRESSION; ADHESION; ALPHA-V-BETA-6; COLLAGEN; BINDING; ALPHA-5-BETA-1;
Keywords:
integrins; cell migration; cell spreading; keratinocyte; fibronectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Larjava, H Univ British Columbia, Fac Dent, 2199 Wesbrook Mall, Vancouver,BC V6T 1Z3, Canada Univ British Columbia 2199 Wesbrook Mall Vancouver BC Canada V6T 1Z3
Citazione:
L. Koivisto et al., "Different integrins mediate cell spreading, haptotaxis and lateral migration of HaCaT keratinocytes on fibronectin", CELL AD COM, 7(3), 1999, pp. 245-257

Abstract

Collaborative role of various fibronectin-binding integrins (alpha 5 beta 1, alpha v beta 1 and alpha v beta 6) as mediators of cell adhesion and migration on fibronectin was studied using cultured HaCaT keratinocytes. This cell line spontaneously expressed all three fibronectin-binding integrins. In addition, the expression of alpha v beta 6 integrin was strongly and specifically upregulated by transforming growth factor-beta 1 (TGF beta 1) whereas the amount of other integrins remained practically unchanged on the cell surface. Adhesion, spreading and motility of HaCaT keratinocytes on fibronectin were promoted by TGF beta 1. Based on antibody blocking experiments, both untreated and TGF beta 1-treated HaCaT cells used alpha v beta 6 integrin as their main fibronectin receptor for cell spreading. In contrast toTGF beta 1-treated cells, the untreated cells also needed alpha 5 beta 1 integrin for maximal cell spreading on fibronectin. Combinations of antibodies blocking both of these receptors totally prevented spreading of both untreated and TGF beta 1-treated cells. Haptotactic motility of individual HaCaT cells through fibronectin-coated membranes was again mainly dependent onalpha v beta 6 integrin, while alpha v beta 1 and alpha 5 beta 1 integrinsplayed a lesser role both in untreated and TGF beta 1-treated HaCaT cells. However, unlike haptotaxis, lateral migration of HaCaT cell sheet was mainly mediated by beta 1 integrins, and alpha v beta 6 integrin showed a minorrole. The migration process appeared to involve a number of beta 1 integrins that could adaptively replace each other when blocking antibodies were present. Thus, keratinocytes appear to use different fibronectin receptors for different functions, such as cell spreading, haptotaxis and lateral migration. The cells can also adapt to a situation where one receptor is unfunctional by switching to another receptor of the same ligand.

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Documento generato il 06/07/20 alle ore 05:49:53