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Titolo:
Maitotoxin and P2Z/P2X(7) purinergic receptor stimulation activate a common cytolytic pore
Autore:
Schilling, WP; Wasylyna, T; Dubyak, GR; Humphreys, BD; Sinkins, WG;
Indirizzi:
Case Western Reserve Univ, Metrohlth Med Ctr, Sch Med, Rammelkamp Ctr Educ& Res, Cleveland, OH 44109 USA Case Western Reserve Univ Cleveland OH USA 44109 Cleveland, OH 44109 USA Case Western Reserve Univ, Metrohlth Med Ctr, Sch Med, Dept Physiol & Biophys, Cleveland, OH 44109 USA Case Western Reserve Univ Cleveland OH USA 44109 Cleveland, OH 44109 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 4, volume: 277, anno: 1999,
pagine: C766 - C776
SICI:
0363-6143(199910)277:4<C766:MAPPRS>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
NONSELECTIVE CATION CHANNEL; P2X(7) NUCLEOTIDE RECEPTOR; CALCIUM INFLUX PATHWAY; CELL-DEATH; CAENORHABDITIS-ELEGANS; EXTRACELLULAR ATP; PLASMA-MEMBRANE; P-2Z RECEPTOR; APOPTOSIS; NEURODEGENERATION;
Keywords:
THP-1 monocytes; HEK cells; heterologous expression; oncosis; vital dyes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Schilling, WP Case Western Reserve Univ, Metrohlth Med Ctr, Sch Med, Rammelkamp Ctr Educ& Res, Rm R322,2500 MetroHlth Dr, Cleveland, OH 44109 USA Case Western Reserve Univ Rm R322,2500 MetroHlth Dr Cleveland OH USA 44109
Citazione:
W.P. Schilling et al., "Maitotoxin and P2Z/P2X(7) purinergic receptor stimulation activate a common cytolytic pore", AM J P-CELL, 277(4), 1999, pp. C766-C776

Abstract

The effects of maitotoxin (MTX) on plasmalemma permeability are similar tothese caused by stimulation of P2Z/P2X(7) ionotropic receptors, suggestingthat 1) MTX directly activates P2Z/P2X(7) receptors or 2) MTX and P2Z/P2X(7) receptor stimulation activate a common cytolytic pore. To distinguish between these two possibilities, the effect of MTX was examined in 1) THP-1 monocytic cells before and after treatment with lipopolysaccharide and interferon-gamma, a maneuver known to upregulate P2Z/P2X(7) receptor, 2) wild-type HEK cells and HEK cells stably expressing the P2Z/P2X(7) receptor, and 3) BW5147.3 lymphoma cells, a cell line that expresses functional P2Z/P2X(7)channels that are poorly linked to pore formation. In control THP-1 monocytes, addition of MTX produced a biphasic increase in the cytosolic free Ca2 concentration ([Ca2+](i)); the initial increase reflects MTX-induced Ca2influx, whereas the second phase correlates in time with the appearance oflarge pores and the uptake of ethidium. MTX produced comparable increases in [Ca2+](i) and ethidium uptake in THP-1 monocytes overexpressing the P2Z/P2X(7) receptor. In both wild-type HEK and HEK cells stably expressing the P2Z/P2X(7) receptor, MTX-induced increases in [Ca2+](i) and ethidium uptakewere virtually identical. The response of BW5147.3 cells to concentrationsof MTX that produced large increases in [Ca2+](i) had no effect on ethidium uptake. In both THP-1 and HEK cells, MTX- and Bz-ATP-induced pores activate with similar kinetics and exhibit similar size exclusion. Last, MTX-induced pore formation, but not channel activation, is greatly attenuated by reducing the temperature to 22 degrees C, a characteristic shared by the P2Z/P2X(7)-induced pore. Together, the results demonstrate that, although MTX activates channels that are distinct from those activated by P2Z/P2X(7) receptor stimulation, the cytolytic/oncotic pores activated by MTX- and Bz-ATP are indistinguishable.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:31:55