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Titolo:
Sulfur mustard upregulates the expression of interleukin-8 in cultured human keratinocytes
Autore:
Lardot, C; Dubois, V; Lison, D;
Indirizzi:
Univ Catholique Louvain, Ind Toxicol & Occupat Med Unit, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium
Titolo Testata:
TOXICOLOGY LETTERS
fascicolo: 1-2, volume: 110, anno: 1999,
pagine: 29 - 33
SICI:
0378-4274(19991029)110:1-2<29:SMUTEO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
NAP-1 IL-8; IRRITANT; CELLS;
Keywords:
sulfur mustard; interleukin-8; human keratinocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Lison, D Univ Catholique Louvain, Ind Toxicol & Occupat Med Unit, Clos Chapelle Champs,Box 30-54, B-1200 Brussels, Belgium Univ Catholique Louvain Clos Chapelle Champs,Box 30-54 Brussels Belgium B-1200
Citazione:
C. Lardot et al., "Sulfur mustard upregulates the expression of interleukin-8 in cultured human keratinocytes", TOX LETT, 110(1-2), 1999, pp. 29-33

Abstract

Although the morphological description of sulfur mustard (SM) injury is well characterised, little is known of the molecular mediators involved in cutaneous toxicity. Since infiltration by lymphocytes and PMNs represents oneof the very first events observed in vivo upon exposure to SM, this study examined whether SM exposure can modify the expression by cultured human keratinocytes of interleukin-8, one of the most important chemoattractants for polymorphonuclear leukocytes (PMNs) in humans. Conditioned medium harvested from control keratinocyte cultures showed a gradual accumulation of thiscytokine over time followed by a levelling off after 12 hours. Upon treatment with 10(-6) and 10(-5) M SM, no significant difference compared to the control situation was observed. After 6 h, a significantly higher amount ofIL-8 was secreted by human keratinocytes treated with 10(-4) M SM and the accumulation of the cytokine persisted up to 24 h after exposure. The expression of IL-8 mRNA was assessed semi-quantitatively (RT-PCR) at the same time points in control and SM-treated (10(-4) M) human keratinocytes. When compared to control cultures, a clear upregulation of IL-8 mRNA levels was observed 6 and 12 h after SM exposure, which is consistent with the secretionpattern of the protein. The present observation indicates that increased secretion of IL-8 by human keratinocytes represents an early event of the inflammatory reaction following SM which is coherent with the reported delay in the recruitment of lymphocytes and PMNs observed in vivo. (C) 1999 Published by Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 18:26:05