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Titolo:
Priming effect of benzo[a]pyrene on monocyte oxidative metabolism: possible mechanisms
Autore:
Fabiani, R; De Bartolomeo, A; Rosignoli, P; Sebastiani, B; Morozzi, G;
Indirizzi:
Univ Perugia, Dept Mol & Cellular Biol, I-06126 Perugia, Italy Univ Perugia Perugia Italy I-06126 Cellular Biol, I-06126 Perugia, Italy
Titolo Testata:
TOXICOLOGY LETTERS
fascicolo: 1-2, volume: 110, anno: 1999,
pagine: 11 - 18
SICI:
0378-4274(19991029)110:1-2<11:PEOBOM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; RESPIRATORY BURST OXIDASE; ALPHA-NAPHTHOFLAVONE; SUPEROXIDE; ACTIVATION; MACROPHAGES; EXPRESSION; RECEPTOR; INVITRO; ENHANCE;
Keywords:
monocytes; polycyclic aromatic hydrocarbons; benzo[a]pyrene; superoxide anion; intracellular calcium; aryl hydrocarbon receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Fabiani, R Univ Perugia, Dept Mol & Cellular Biol, Via Giochetto, I-06126 Perugia, Italy Univ Perugia Via Giochetto Perugia Italy I-06126 erugia, Italy
Citazione:
R. Fabiani et al., "Priming effect of benzo[a]pyrene on monocyte oxidative metabolism: possible mechanisms", TOX LETT, 110(1-2), 1999, pp. 11-18

Abstract

Monocytes, separated from human peripheral blood, were preincubated with different polycyclic aromatic hydrocarbons (PAHs) for 24 h and the production of superoxide ions (O-2(.-)) was then measured using as a stimulating agent phorbol 12-myristate 13-acetate. A significantly enhanced O-2(.-) production is only observed when the cells are treated with benzo[a]pyrene (B[a]P); benzo[e]pyrene, benzo[a]anthracene and 3-methylcholanthrene induce a small but not significant increase of O-2(.-) Anthracene has no effect, while phenanthrene slightly inhibits. The priming activity of B[a]P is unrelated to variations in intracellular Ca2+ ([Ca2+](i)), as demonstrated by the inability of B[a]P to increase [Ca2+](i) concentration in both monocytes and the promonocytic cell line U937. Furthermore, in monocytes the sarcoplasmic/endoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin, which can increase [Ca2+](i) evokes a differentiation-like event associated with a decreasein the production of superoxide ions. These results further support that the enhancing activity of B[a]P on monocytes superoxide production is not mediated by an increase of [Ca2+](i). In contrast, the role of the aryl hydrocarbon receptor (AhR) in B[a]P-induced superoxide ion enhancement is suggested by the inhibitory effect of the specific antagonist alpha-naphthoflavone (alpha NF), while the tumor necrosis factor (TNF-alpha) is not involved in the phenomenon. Thus, the interaction of B[a]P with its cytosolic receptor and either the metabolism of the compound into reactive intermediates or the over-expression of some unknown genes seem to be involved in an essential step in this process. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:43:30