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Titolo:
A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures
Autore:
Miller, CA;
Indirizzi:
Tulane Univ, Sch Publ Hlth & Trop Med, Dept Environm Hlth Sci, New Orleans, LA 70112 USA Tulane Univ New Orleans LA USA 70112 Hlth Sci, New Orleans, LA 70112 USA Tulane Xavier Ctr Bioenvironm Res, New Orleans, LA 70112 USA Tulane XavierCtr Bioenvironm Res New Orleans LA USA 70112 , LA 70112 USA
Titolo Testata:
TOXICOLOGY AND APPLIED PHARMACOLOGY
fascicolo: 3, volume: 160, anno: 1999,
pagine: 297 - 303
SICI:
0041-008X(19991101)160:3<297:AHAHRS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEIN; AH RECEPTOR; IN-VIVO; EXPRESSION; COMPLEX; DIOXIN; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN; ACTIVATION; TOXICITY; SYSTEM;
Keywords:
yeast; aryl hydrocarbon receptor; aryl hydrocarbon receptor nuclear translocator; dioxin; aromatic hydrocarbon; ligand mixtures; hexachlorobenzene; benzo(a)pyrene; beta-napthoflavone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, CA Tulane Univ, Sch Publ Hlth & Trop Med, Dept Environm Hlth Sci, 1430 TulaneAve, New Orleans, LA 70112 USA Tulane Univ 1430 Tulane Ave New Orleans LA USA 70112 70112 USA
Citazione:
C.A. Miller, "A human aryl hydrocarbon receptor signaling pathway constructed in yeast displays additive responses to ligand mixtures", TOX APPL PH, 160(3), 1999, pp. 297-303

Abstract

An optimized signal transduction pathway that reproduces the response of human aryl hydrocarbon (Ah) receptor to ligands has been established in Saccharomyces cerevisiae. Ligand treatment induced a 50-fold increase in beta-galactosidase activity from a reporter plasmid in yeast engineered to express human Ah receptor and Ah nuclear translocator (Arnt) proteins. The archetypal Ah receptor ligand, 2,3,7,8-tetrachlorodibenzo(p)dioxin, activated Ah receptor and induced lacZ reporter activity at concentrations of greater than or equal to 0.3 nM. Mixtures of halogenated and nonhalogenated Ah receptor ligands produced additive signaling responses in this yeast bioassay. These results were consistent with the existence of a common binding site andmechanism of ligand-mediated Ah receptor activation. Although yeast have no natural counterpart to the Ah receptor pathway, expression of human Ah receptor and Arnt under the appropriate conditions provides a functional model system for studying Ah receptor activation and signal transduction. (C) 1999 Academic Press.

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Documento generato il 25/11/20 alle ore 15:24:59