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Titolo:
Beryllium-stimulated in vitro migration of peripheral blood lymphocytes
Autore:
Sawyer, RT; Doherty, DE; Schumacher, BA; Newman, LS;
Indirizzi:
Natl Jewish Med & Res Ctr, Dept Med, Div Environm & Occupat Hlth Sci, Denver, CO 80206 USA Natl Jewish Med & Res Ctr Denver CO USA 80206 h Sci, Denver, CO 80206 USA Univ Colorado, Hlth Sci Ctr, Dept Med, Div Pulm Sci & Crit Care Med, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 i & Crit Care Med, Denver, CO 80262 USA Univ Kentucky, Albert B Chandler Med Ctr, Div Pulm & Crit Care Med, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 it Care Med, Lexington, KY 40536 USA Univ Colorado, Hlth Sci Ctr, Dept Prevent Med & Biometr, Denver, CO 80262 USA Univ Colorado Denver CO USA 80262 ent Med & Biometr, Denver, CO 80262 USA
Titolo Testata:
TOXICOLOGY
fascicolo: 3, volume: 138, anno: 1999,
pagine: 155 - 163
SICI:
0300-483X(19991115)138:3<155:BIVMOP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHEMOTACTIC FACTORS; NATURAL-HISTORY; DISEASE; SENSITIZATION; INFLAMMATION; MONOCYTE; BINDING; SUBSETS;
Keywords:
beryllium; blood lymphocytes; blood monocytes; peripheral blood mononuclear cells; cell migration; chronic beryllium disease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Sawyer, RT Natl Jewish Med & Res Ctr, Dept Med, Div Environm & Occupat Hlth Sci, Denver, CO 80206 USA Natl Jewish Med & Res Ctr Denver CO USA 80206 er, CO 80206 USA
Citazione:
R.T. Sawyer et al., "Beryllium-stimulated in vitro migration of peripheral blood lymphocytes", TOXICOLOGY, 138(3), 1999, pp. 155-163

Abstract

Inhalation of beryllium (Be) induces both inflammatory and metal antigen-specific immune responses in the lungs characterized by mononuclear cell infiltration and granuloma formation (chronic beryllium disease, CBD). We tested the hypothesis that Be-salts might increase the in vitro migration of peripheral blood mononuclear cells (PBMC). PBMC are mixed cells, consisting of lymphocytes and monocytes. We compared their responses to populations of both purified blood lymphocytes, and purified blood monocytes. Purified blood monocytes and lymphocytes, isolated by Percoll gradients and centrifugalelutriation from normal human subjects (n = 6), were exposed to graded concentrations (0.01 to 100 mu M) of BeSO4 or to the control metal-salt Al-2(SO4)(3). Migratory responses of stimulated PBMC were measured in Boyden Chambers. As controls, PBMC mixed cells or purified lymphocytes or purified monocytes were unstimulated or stimulated with a positive chemoattractant, Zymosan-A treated pooled, normal human serum (ZAS). The migration index (MI) was defined as the distance (micrometers) that cells migrated through a 5 mufilter. The MI for unstimulated PBMC mixed cells was 75 +/- 4 whereas the MI for ZAS-stimulated PBMC mixed cells was 124 +/- 4 (P less than or equal to 0.05, Tukey-Kramer). The MI for BeSO4 -stimulated (100 mu M) PBMC mixed cells was 136 +/- 4. The observed increase in the BeSO4-stimulated PBMC mixed cell migration was significant down to 0.1 mu M BeSO4. BeSO4, BeCl2 and BeF2, tested at 100 and 10 mu M, were equally effective at inducing PBMC mixed cell migration. Equimolar concentrations of Al-2(SO4)(3) were not as effective at inducing PBMC mixed cell migration, MI < 100 at 100 mu M, and did not induce PBMC mixed cell migration at concentrations below 1 mu M The migration of purified monocytes through filters was not increased in response to either BeSO4 or Al-2(SO4)(3), compared to controls, but did respond toZAS (MI = 100 +/- 4). Purified lymphocytes migrated in response to stimulation with all concentrations of BeSO4 tested (100 mu M MI = 133 +/- 9), andAl-2(SO4)(3) (100 mu M MI = 85 +/- 8). There were no significant differences in the MI for PBMC mixed cells or for purified lymphocytes at the concentrations of BeSO4 tested. Our data show that Be directly induces the in vitro migration of PBMC mixed cells and purified blood lymphocytes, and not purified blood monocytes, across a broad range of Be concentrations. This induction of migration was independent of the molecular form of the Be-salt. Inhaled Be, by promoting lymphocyte emigration to the lung, may create a microenvironment that favors a Be-antigen-specific T-lymphocyte response, chronic inflammation, and CBD. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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Documento generato il 20/01/21 alle ore 02:58:32