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Titolo:
Specificity in transforming growth factor beta-induced transcription of the plasminogen activator inhibitor-1 gene: Interactions of promoter DNA, transcription factor mu E3, and Smad proteins
Autore:
Hua, XX; Miller, ZA; Wu, G; Shi, YG; Lodish, HF;
Indirizzi:
Whitehead Inst Biomed Res, Cambridge, MA 02142 USA Whitehead Inst Biomed Res Cambridge MA USA 02142 Cambridge, MA 02142 USA MIT, Dept Biol, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139MIT, Dept Biol, Cambridge, MA 02139 USA Princeton Univ, Lewis Thomas Lab, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 t Mol Biol, Princeton, NJ 08544 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 23, volume: 96, anno: 1999,
pagine: 13130 - 13135
SICI:
0027-8424(19991109)96:23<13130:SITGFB>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TGF-BETA; BINDING-PROTEIN; TFE3; PHOSPHORYLATION; RESPONSES; RECEPTOR; COOPERATION; COACTIVATOR; FAST-1; DOMAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Lodish, HF Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142USA Whitehead Inst Biomed Res 9 Cambridge Ctr Cambridge MA USA 02142
Citazione:
X.X. Hua et al., "Specificity in transforming growth factor beta-induced transcription of the plasminogen activator inhibitor-1 gene: Interactions of promoter DNA, transcription factor mu E3, and Smad proteins", P NAS US, 96(23), 1999, pp. 13130-13135

Abstract

Transforming growth factor beta (TCF-beta) regulates a broad range of biological processes, including cell growth, development, differentiation, and immunity. TCF-beta signals through its cell surface receptor serine kinasesthat phosphorylate Smad2 or Smad3 proteins. Because Smad3 and its partner Smad4 bind to only 4-bp Smad binding elements (SBEs) in DNA, a central question is how specificity of TCF-beta-induced transcription is achieved. We show that Smad3 selectively binds to two of the three SBEs in PE2.1, a TGF-beta-inducible fragment of the plasminogen activator inhibitor-1 promoter, to mediate TGF-beta-induced transcription; moreover, a precise 3-bp spacer between one SEE and the E-box, a binding site for transcription factor mu E3(TFE3), is essential for TCF-beta-induced transcription. Whereas an isolated Smad3 MH1 domain binds to TFE3, TGF-beta receptor-mediated phosphorylation of full-length Smad3 enhances its binding to TFE3. Together, these studies elucidate an important mechanism for specificity in TGF-beta-induced transcription of the plasminogen activator inhibitor-1 gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 13:05:11