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Titolo:
Optical detection of cytochrome P450 by sensitizer-linked substrates
Autore:
Dmochowski, IJ; Crane, BR; Wilker, JJ; Winkler, JR; Gray, HB;
Indirizzi:
CALTECH, Beckman Inst, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 LTECH, Beckman Inst, Pasadena, CA 91125 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 23, volume: 96, anno: 1999,
pagine: 12987 - 12990
SICI:
0027-8424(19991109)96:23<12987:ODOCPB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVE-SITE PROBES; CRYSTAL-STRUCTURE; COMPLEXES; P450(CAM);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Crane, BR CALTECH, Beckman Inst, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 kman Inst, Pasadena, CA 91125 USA
Citazione:
I.J. Dmochowski et al., "Optical detection of cytochrome P450 by sensitizer-linked substrates", P NAS US, 96(23), 1999, pp. 12987-12990

Abstract

The ability to detect, characterize, and manipulate specific biomolecules in complex media is critical for understanding metabolic processes. Particularly important targets are oxygenases (cytochromes P450) involved in drug metabolism and many disease states, including liver and kidney dysfunction,neurological disorders, and cancer. We have found that Ru photosensitizerslinked to P450 substrates specifically recognize submicromolar cytochrome P450(cam) in the presence of other heme proteins. In the P450:Ru-substrate conjugates, energy transfer to the heme dramatically accelerates the Ru-luminescence decay, The crystal structure of a P450(cam):Ru-adamantyl complex reveals access to the active center via a channel whose depth (Ru-Fe distance is 21 Angstrom) is virtually the same as that extracted from an analysisof the energy-transfer kinetics. Suitably constructed libraries of sensitizer-linked substrates could be employed to probe the steric and electronic properties of buried active sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:07:20