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Titolo:
The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland ductal cell line HSG cells
Autore:
Morimoto, Y; Morimoto, H; Kobayashi, S; Ohba, T; Haneji, T;
Indirizzi:
Kyushu Dent Coll, Dept Oral Anat 1, Kokurakita Ku, Kitakyushu, Fukuoka 8038580, Japan Kyushu Dent Coll Kitakyushu Fukuoka Japan 8038580 Fukuoka 8038580, Japan Kyushu Dent Coll, Dept Dent Radiol, Kokurakita Ku, Kitakyushu, Fukuoka 8038580, Japan Kyushu Dent Coll Kitakyushu Fukuoka Japan 8038580 Fukuoka 8038580, Japan
Titolo Testata:
ORAL DISEASES
fascicolo: 2, volume: 5, anno: 1999,
pagine: 104 - 110
SICI:
1354-523X(199904)5:2<104:TPPIOA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SALIVARY-GLAND; NEOPLASTIC EPITHELIAL DUCT; DEATH; EXPRESSION; ACTIVATION;
Keywords:
apoptosis; HSG cells; salivary gland; okadaic acid; calyculin A; protein phosphatase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Haneji, T Kyushu Dent Coll, Dept Oral Anat 1, Kokurakita Ku, 2-6-1 Manazuru, Kitakyushu, Fukuoka 8038580, Japan Kyushu Dent Coll 2-6-1 Manazuru Kitakyushu Fukuoka Japan 8038580
Citazione:
Y. Morimoto et al., "The protein phosphatase inhibitors, okadaic acid and calyculin A, induce apoptosis in human submandibular gland ductal cell line HSG cells", ORAL DIS, 5(2), 1999, pp. 104-110

Abstract

OBJECTIVE: To investigate a possible relationship between protein phosphorylation or dephosphorylation status and apoptosis in salivary gland cells, we examined the effects of okadaic acid and calyculin A, the protein phosphatase inhibitors, on cultured human submandibular gland ductal cell line, HSG cells. METHODS: HSG cells at subconfluent stages were exposed to varying concentrations of okadaic acid or calyculin A. Apoptoses were analysed in HSG cellsby phase-contrast microscopy, WST-1 cytotoxicity assay, Hoechst 33342 staining, and DNA ladder formation. RESULT: Both okadaic acid and calyculin A induced cell death in HSG cells in a dose-dependent fashion. Marked nuclear condensation and fragmentation of chromatin was observed in HSG cells. DNA ladder formation was also detected in HSG cells by treatment with okadaic acid or calyculin A. The inducedDNA ladder formation was dose-dependent with maximal effect at concentrations of 50 nM okadaic acid and 2 nM calyculin A, respectively, and were time-dependent from 14 h to 48 h. To further determine if new gene transcription and protein synthesis regulate okadaic acid-induced apoptosis in HSG cells, the cells were treated with cycloheximide or actinomycin D in the presence of 20 nM okadaic acid. Neither inhibitor protected the cells against okadaic acid-induced apoptosis. CONCLUSION: Based on the known selectivity of okadaic acid and calyculin A, our results indicate that the pathway of the apoptosis in the cultured salivary gland cells is regulated by protein phosphatase type 1 or type 2A. Our results also suggest that new protein synthesis and/or mRNA expression are not involved in okadaic acid-induced apoptosis in HSG cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:11:57