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Titolo:
Expression of cytotoxicity by potential pathogens in the standard Escherichia coli collection of reference (ECOR) strains
Autore:
Lai, XH; Wang, SY; Uhlin, BE;
Indirizzi:
Umea Univ, Dept Microbiol, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 Univ, Dept Microbiol, S-90187 Umea, Sweden Chinese Acad Prevent Med, Inst Epidemiol & Microbiol, Minist Publ Hlth, Prior Lab Mol Med Bacteriol, Beijing, Peoples R China Chinese Acad Prevent Med Beijing Peoples R China ijing, Peoples R China
Titolo Testata:
MICROBIOLOGY-UK
, volume: 145, anno: 1999,
parte:, 11
pagine: 3295 - 3303
SICI:
1350-0872(199911)145:<3295:EOCBPP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMID-ENCODED HEMOLYSIN; HORIZONTAL GENE-TRANSFER; ALPHA-HEMOLYSIN; NATURAL-POPULATIONS; NUCLEOTIDE-SEQUENCE; DIARRHEA; TOXIN; O157-H7; ISLAND; DNA;
Keywords:
cytotoxicity; haemolysin; enteroaggregative Escherichia coli; enteroaggregative heat-stable enterotoxin; ECOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Lai, XH Umea Univ, Dept Microbiol, S-90187 Umea, Sweden Umea Univ Umea Sweden S-90187 pt Microbiol, S-90187 Umea, Sweden
Citazione:
X.H. Lai et al., "Expression of cytotoxicity by potential pathogens in the standard Escherichia coli collection of reference (ECOR) strains", MICROBIO-UK, 145, 1999, pp. 3295-3303

Abstract

The standard Escherichia coli collection of reference (ECOR) strains was examined for ability to exert cytotoxicity towards mammalian cells. A group of strains with functional haemolysin expression caused strong cytotoxicityand detachment in J774 macrophage cells as measured by lactate dehydrogenase release and as observed under a microscope. The expression of haemolysinwas monitored by using antisera recognizing the E, coli alpha-haemolysin, the HlyA protein, and by quantitative haemolysis assays. The presence of the hlyA gene, which may be part of a pathogenicity island, was also confirmed. These analyses revealed that different ECOR strains express quantitatively different levels of haemolysin. One putative enteroaggregative E, coli (EAEC) strain was also found in the ECOR collection. The EAEC strain was characterized by the clump formation assay, PCR amplification of the EAEC DMA probe sequence and confirmative sequence analysis of the amplified fragment. The EAEC heat-stable enterotoxin 1 gene, astA, was found in 14% (10/72) of the ECOR strains and a consensus sequence for astA was proposed by comparing these sequences with those from pathogens. The astA gene appeared to beplasmid-located, Based on evidence from the work of other laboratories andfrom the present findings, it is concluded that the ECOR collection contains strains that may represent pathogenic E, coli, It is noted that caution is necessary when handling or disposing of those potentially pathogenic ECOR strains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:41:50