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Titolo:
Mode of Ca2+ action on ciliary beat frequency in single ovine airway epithelial cells
Autore:
Salathe, M; Bookman, RJ;
Indirizzi:
Univ Miami, Sch Med, Dept Mol & Cellular Pharmacol, Miami, FL 33136 USA Univ Miami Miami FL USA 33136 l & Cellular Pharmacol, Miami, FL 33136 USA Univ Miami, Sch Med, Div Pulm & Crit Care Med, Miami, FL 33136 USA Univ Miami Miami FL USA 33136 v Pulm & Crit Care Med, Miami, FL 33136 USA
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 3, volume: 520, anno: 1999,
pagine: 851 - 865
SICI:
0022-3751(19991101)520:3<851:MOCAOC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN RESPIRATORY EPITHELIUM; TRACHEAL EPITHELIUM; MICROTUBULE TRANSLOCATION; CALCIUM REGULATION; P2X(7) RECEPTOR; 22S DYNEIN; IN-VITRO; PARAMECIUM; INVITRO; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Bookman, RJ Univ Miami, Sch Med, Dept Mol & Cellular Pharmacol, 1600 NW 10th Ave,Room 6056 R-189, Miami, FL 33136 USA Univ Miami 1600 NW 10th Ave,Room 6056 R-189 Miami FL USA 33136
Citazione:
M. Salathe e R.J. Bookman, "Mode of Ca2+ action on ciliary beat frequency in single ovine airway epithelial cells", J PHYSL LON, 520(3), 1999, pp. 851-865

Abstract

1. We analysed the kinetics of coupling between cytoplasmic calcium ([Ca2+](i)) and ciliary beat frequency (CBF) using simultaneous single cilium recording and single cell [Ca2+](i) measurements from cultured ovine tracheal epithelial cells.2. CBF and [Ca2+](i) (indicated by fura-2) were measured at rest and in response to activation of the G-protein coupled M3 muscarinic receptor by 10 mu M acetylcholine (ACh).3. Fourier transform analysis of 3s data segments of light intensity from phase-contrast microscopy showed no significant delay between changes in [Ca2+](i) and CBF during a 2 min exposure to ACh and subsequent washout.4. CBF time resolution was improved by computing instantaneous beat frequency. This revealed that CBF lagged the rapid increase in [Ca2+](i) in response to ACh with a delay of less than 1 beat cycle (143 ms at 7 Hz). When CBF was estimated by an improved Fourier method, this delay was observed to be 70 +/- 30 ms (mean +/- S.E.M.; n = 20 cilia). During the slower return tobaseline, a lag of 8 +/- 3.2 s was observed, indicative of hysteresis.5. While calmodulin inhibitors (calmidazolium and W-7; each n = 5) decreased baseline CBF by an average of 1.1 +/- 0.1 Hz, they did not alter the kinetic relationship between [Ca2+](i) and CBF. Similarly, phosphatase inhibitors (okadaic acid and cyclosporin A; each n = 5), changed neither baseline CBF nor the kinetic coupling between [Ca2+](i) and CBF.6. These data suggest that the timing of Ca2+ action on CBF in ovine airway epithelial cells, is unlikely to be determined by phosphorylation reactions involving calmodulin or kinase/phosphatase reactions.7. A simple model for Ca2+ stimulation of CBF is presented. Pits of the. model to the data suggest four or more Ca2+ ions bind cooperatively to speedup CBF.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:37:49