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Titolo:
Interactive role for neurosteroids in ethanol enhancement of gamma-aminobutyric acid-gated currents from dissociated substantia nigra reticulata neurons
Autore:
Criswell, HE; McCown, TJ; Ming, Z; Mueller, RA; Breese, GR;
Indirizzi:
Univ N Carolina, Sch Med, Bowles Ctr Alcohol Studies, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 udies, Chapel Hill, NC 27599 USA Univ N Carolina, Ctr Neurosci, Dept Psychiat, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA i, Dept Psychiat, Chapel Hill, NC USA Univ N Carolina, Ctr Neurosci, Dept Anesthesiol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA Dept Anesthesiol, Chapel Hill, NC USA Univ N Carolina, Ctr Neurosci, Dept Pharmacol, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA , Dept Pharmacol, Chapel Hill, NC USA Univ N Carolina, Sch Med, Gene Therapy Ctr, Chapel Hill, NC 27599 USA UnivN Carolina Chapel Hill NC USA 27599 y Ctr, Chapel Hill, NC 27599 USA
Titolo Testata:
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
fascicolo: 3, volume: 291, anno: 1999,
pagine: 1054 - 1059
SICI:
0022-3565(199912)291:3<1054:IRFNIE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GABA(A) RECEPTOR FUNCTION; MEDIAL SEPTAL AREA; RAT-BRAIN SLICES; INDIVIDUAL NEURONS; CORTICAL-NEURONS; INHIBITION; MODULATION; ZOLPIDEM; CHANNELS; POTENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Criswell, HE Univ N Carolina, Sch Med, Bowles Ctr Alcohol Studies, Thurston Bowles BldgCB 7178, Chapel Hill, NC 27599 USA Univ N Carolina Thurston Bowles Bldg CB 7178 Chapel Hill NC USA 27599
Citazione:
H.E. Criswell et al., "Interactive role for neurosteroids in ethanol enhancement of gamma-aminobutyric acid-gated currents from dissociated substantia nigra reticulata neurons", J PHARM EXP, 291(3), 1999, pp. 1054-1059

Abstract

Although previous in vivo electrophysiological studies demonstrated a consistent ethanol enhancement of gamma-aminobutyric acid (GABA) responsivenessfrom substantia nigra reticulata (SNR) neurons, ethanol applied in vitro to dissociated neurons from the SNR had an inconsistent effect on GABA function. One source for the disparity between these contrasting in vivo and in vitro results could be an endogenous factor (acting on an auxiliary site onGABA(A) receptors) that was not available to the isolated SNR neurons. Because neurosteroids are present in vivo and act on an auxiliary site, it washypothesized that the presence of a neurosteroid was important for a consistent effect of ethanol on GABA responsiveness from neurons studied in vitro. Alone, the neurosteroid analog alphaxalone produced a significant, concentration-related enhancement of GABA responsiveness from isolated SNR neurons. In contrast to an inconsistent action of 100 mM ethanol on GABA responsiveness in the absence of alphaxalone, the presence of 30 and 100 nM alphaxalone resulted in the majority of isolated neurons responding to this ethanol level. At a concentration of alphaxalone as low as 30 nM, ethanol produced a robust concentration- related increase in GABA-gated currents from this cell type. The neurosteroid 3 alpha,5 alpha-tetrahydrodeoxycorticosterone(100 nM) also permitted a reliable concentration- dependent ethanol enhancement of responses to GABA from SNR cells, indicative that the effects of alphaxalone were not unique. This consistent neurosteroid-induced ethanol enhancement of GABA responsiveness from dissociated SNR neurons supports the view that neurosteroids may play a key role in the action of ethanol on postsynaptic GABA(A) receptor function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 10:16:12