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Titolo:
Vesicular monoamine transporter 1 is responsible for storage of 5-hydroxytryptamine in rat pinealocytes
Autore:
Hayashi, M; Haga, M; Yatsushiro, S; Yamamoto, A; Moriyama, Y;
Indirizzi:
Okayama Univ, Fac Pharmaceut Sci, Dept Biochem, CREST,Japan Sci & Technol Corp, Okayama 7008530, Japan Okayama Univ Okayama Japan 7008530 Technol Corp, Okayama 7008530, Japan Kansai Med Univ, Dept Physiol, Moriguchi, Osaka 570, Japan Kansai Med Univ Moriguchi Osaka Japan 570 ol, Moriguchi, Osaka 570, Japan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 6, volume: 73, anno: 1999,
pagine: 2538 - 2545
SICI:
0022-3042(199912)73:6<2538:VMT1IR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOVINE POSTERIOR PITUITARY; DENSE CORE VESICLES; MELATONIN SYNTHESIS; L-GLUTAMATE; EXTRACELLULAR SEROTONIN; CHEMICAL TRANSDUCTION; CHROMAFFIN GRANULES; GLANDS INVITRO; MICROVESICLES; RELEASE;
Keywords:
pinealocytes; vesicular monoamine transporter (VMAT); 5-hydroxytryptamine; exocytosis; melatonin; microvesicles;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Moriyama, Y Okayama Univ, Fac Pharmaceut Sci, Dept Biochem, CREST,Japan Sci & Technol Corp, Okayama 7008530, Japan Okayama Univ Okayama Japan 7008530 p, Okayama 7008530, Japan
Citazione:
M. Hayashi et al., "Vesicular monoamine transporter 1 is responsible for storage of 5-hydroxytryptamine in rat pinealocytes", J NEUROCHEM, 73(6), 1999, pp. 2538-2545

Abstract

Vesicular monoamine transporters (VMATs) are involved in chemical transduction in monoaminergic neurons and various endocrine cells through the storage of monoamines in secretory vesicles. Mammalian pinealocytes contain more5-hydroxytryptamine (5-HT) than any other cells and are expected to contain VMAT, although no information is available so far. Upon the addition of ATP, radiolabeled 5-HT was taken up by a particulate fraction prepared from cultured rat pinealocytes. The 5-HT uptake was inhibited significantly by bafilomycin A(1) (an inhibitor of vacuolar H+-ATPase), 3,5-di-tert-butyl-4-hydroxybenzylidenemalononitrile (a proton conductor), or reserpine (an inhibitor of VMAT). RT-PCR analysis suggested that VMAT type 1 (VMAT1), but not type 2, is expressed. Antibodies against VMAT1 recognized a single polypeptide with an apparent molecular mass of similar to 55 kDa, and specifically immunostained pinealocytes. VMAT1 immunoreactivity was high in the vesicular structures in the Varicosities of long branching processes and was associated with 5-HT, but not with synaptophysin, a marker protein for microvesicles. The 5-HT immunoreactivity in the long branching processes disappeared upon incubation with reserpine. These results indicate that 5-HT, at least in part, is stored in vesicles other than microvesicles in pinealocytes through a mechanism similar to that of various secretory vesicles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 05:14:57