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Titolo:
Potential for aging female Aedes aegypti (Diptera : Culicidae) by gas chromatographic analysis of cuticular hydrocarbons, including a field evaluation
Autore:
Desena, ML; Clark, JM; Edman, JD; Symington, SB; Scott, TW; Clark, GG; Peters, TM;
Indirizzi:
Univ Massachusetts, Dept Entomol, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 pt Entomol, Amherst, MA 01003 USA
Titolo Testata:
JOURNAL OF MEDICAL ENTOMOLOGY
fascicolo: 6, volume: 36, anno: 1999,
pagine: 811 - 823
SICI:
0022-2585(199911)36:6<811:PFAFAA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGE; SEX; TEMPERATURE; LIPIDS;
Keywords:
Aedes aegypti; age-grading; cuticular hydrocarbons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Desena, ML 38th Med Detachment PM, Unit 15684, APO AP 96203, Seoul, South Korea 38th Med Detachment PM APO AP 96203 Seoul South Korea h Korea
Citazione:
M.L. Desena et al., "Potential for aging female Aedes aegypti (Diptera : Culicidae) by gas chromatographic analysis of cuticular hydrocarbons, including a field evaluation", J MED ENT, 36(6), 1999, pp. 811-823

Abstract

Gas chromatography with flame-ionization detection was used to measure thetime-associated, quantitative changes in the cuticular hydrocarbons of female Aedes aegypti (L.). Cohorts of unstressed Ac, aegypti, Rockefeller strain, were reared and held at 3 constant temperatures (24, 28, and 30 degreesC). Five females from each cohort were taken at 33 degree-day (DD) intervals from 0 to 231 DD (using 17 degrees C as the threshold temperature). Quantitative changes over time of cuticular hydrocarbons associated with gas chromatographic peaks 1 and 5 were identified as having promise for age grading. The relative abundance of peak 1 (pentacosane) decreased linearly from 0 to 132 DD, whereas peak 5 (nonacosane) increased linearly over the same period. Suboptimal larval conditions (crowded and starved), which resulted in physiological stress (decreased size), had negligible effect on the relative abundance of pentacosane and nonacosane. Additionally, the rate of change in the relative abundance of pentacosane and nonacosane were the same for both a recently colonized Chachoengsao (Thailand) strain of Ae. aegypti compared with the long-colonized Rockefeller (Caribbean) strain over a 0-99 DD interval. Two linear regression models, one based on the relative abundance of pentacosane and the other on the legit transformation of these values, were developed for aging female Ae. aegypti. A blind study using laboratory-reared mosquitoes and a mark-release-recapture experiment using field mosquitoes validated these age-grading models and produced promising resultsfor aging females up to 132 DD (19, 12, and 10 calendar days at 24, 28 and30 degrees C, respectively). Therefore the regression models, based on therelative abundance of these 2 cuticular hydrocarbons, appeared to be a useful approach for age-grading Ae. aegypti up to at least 12 d of age regardless of environmental conditions (temperature and stress) and population history (origin and colonization time).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 02:47:12