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Titolo:
High performance liquid chromatographic method for the quantification of putrescine and cadaverine as their electroactive derivatives: Application tobiological samples
Autore:
Bousquet, E; Acquaviva, R; Spadaro, A; Vanella, A;
Indirizzi:
Univ Catania, Fac Farm, Dipartimento Sci Farmaceut, I-95125 Catania, ItalyUniv Catania Catania Italy I-95125 Sci Farmaceut, I-95125 Catania, Italy Univ Catania, Ist Chim Biol, Fac Farm, I-95125 Catania, Italy Univ Catania Catania Italy I-95125 iol, Fac Farm, I-95125 Catania, Italy
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 19, volume: 22, anno: 1999,
pagine: 2957 - 2974
SICI:
1082-6076(1999)22:19<2957:HPLCMF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORTHO-PHTHALALDEHYDE; ELECTROCHEMICAL DETECTION; PRECOLUMN DERIVATIZATION; MONOACETYL DERIVATIVES; AMINO-ACIDS; POLYAMINES; DIAMINES; ASSAY; SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Bousquet, E Univ Catania, Fac Farm, Dipartimento Sci Farmaceut, Vle A Doria 6, I-95125Catania, Italy Univ Catania Vle A Doria 6 Catania Italy I-95125 tania, Italy
Citazione:
E. Bousquet et al., "High performance liquid chromatographic method for the quantification of putrescine and cadaverine as their electroactive derivatives: Application tobiological samples", J LIQ CHR R, 22(19), 1999, pp. 2957-2974

Abstract

A high performance liquid chromatographic method coupled with an electrochemical detector for the separation and quantification of putrescine and cadaverine as their electroactive derivatives has been developed. Two different solvent column systems were used to determine putrescine, cadaverine, spermidine, spermine, and 1,3-diaminopropane (IS) in rat cerebral cortex. The lower detection limit was less than 80 fmol. The electrochemical response was linear in the range 80-240 ng/mL. These chromatographic procedures are suitable for quantification of putrescine and cadaverine in animal tissues. Because of the simplicity, specificity, sensitivity, and ease of the operations, the method is applicable in both research and clinical laboratories.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:41:13