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Titolo:
Carbazole-9-N-acetyl-N-hydroxysuccinimide (CAHS) as precolumn derivatization agent for fluorimetric detection of amino compounds with liquid chromatography
Autore:
You, JM; Lao, WJ; Sun, XJ; Ou, QY;
Indirizzi:
Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples R China Chinese Acad Sci Lanzhou Peoples R China 730000 730000, Peoples R China Qufu Normal Univ, Dept Chem, Shandong 273165, Peoples R China Qufu Normal Univ Shandong Peoples R China 273165 273165, Peoples R China
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 19, volume: 22, anno: 1999,
pagine: 2907 - 2923
SICI:
1082-6076(1999)22:19<2907:C(APD>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE DETECTION; ALIPHATIC-AMINES; ULTRAVIOLET DETECTION; REAGENT; ACIDS; POLYAMINES; CHLOROFORMATE; METHANOL; WATER; GAS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Ou, QY Chinese Acad Sci, Lanzhou Inst Chem Phys, Lanzhou 730000, Peoples RChina Chinese Acad Sci Lanzhou Peoples R China 730000 , Peoples R China
Citazione:
J.M. You et al., "Carbazole-9-N-acetyl-N-hydroxysuccinimide (CAHS) as precolumn derivatization agent for fluorimetric detection of amino compounds with liquid chromatography", J LIQ CHR R, 22(19), 1999, pp. 2907-2923

Abstract

A simple and sensitive LC method for the determination of amino compounds,using carbazole-N-acetyl-N-hydroxysuccinimide (CAHS) as condensation agent, has been developed. A mixture of amines is treated with CAHS, in the presence of triethylamine catalyst in non-aqueous acetonitrile or in the presence of 0.2 M berate buffer at pH 8.0-9.0 in 40% (v/v) of acetonitrile solution, to give quantitative yields of amides. Emission maximum for the derivatized amines is 365 nm (lambda(ex) 335 nm). Studies on derivatization conditions indicate that amines react very fast with CAHS under proposed conditions. The method, in conjunction with a multi-step gradient, offers baseline resolution of common amine derivatives on a reversed-phase C-18 column. Derivatization reaction is more convenient and more efficient than previous methods which require prior conversion of carboxylic acids to acyl chlorides. The LC separation of amine derivatives has good reproducibility. The established method is also suitable for the determination of other amines invarious biological fluids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 18:40:17