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Titolo:
Enhancement of antibody responses by DNA immunization using expression vectors mediating efficient antigen secretion
Autore:
Svanholm, C; Bandholtz, L; Lobell, A; Wigzell, H;
Indirizzi:
Karolinska Inst, Ctr Microbiol & Tumor Biol, S-17177 Stockholm, Sweden Karolinska Inst Stockholm Sweden S-17177 Biol, S-17177 Stockholm, Sweden
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 228, anno: 1999,
pagine: 121 - 130
SICI:
0022-1759(19990831)228:1-2<121:EOARBD>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMID DNA; AUTOIMMUNE ENCEPHALOMYELITIS; PROTECTIVE IMMUNITY; BACTERIAL-DNA; CPG MOTIFS; T-CELL; VACCINATION; GENE; PROTEIN; VIRUS;
Keywords:
DNA immunization; signal sequence; nef; omp2; antibody response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Svanholm, C Karolinska Inst, Ctr Microbiol & Tumor Biol, Box 280, S-17177 Stockholm, Sweden Karolinska Inst Box 280 Stockholm Sweden S-17177 holm, Sweden
Citazione:
C. Svanholm et al., "Enhancement of antibody responses by DNA immunization using expression vectors mediating efficient antigen secretion", J IMMUNOL M, 228(1-2), 1999, pp. 121-130

Abstract

The immune responses elicited in mice, after intradermal (i.d.) immunization with plasmids encoding secreted or intracellular forms of HIV-1 nef, HIV-1 rat or C. pneumoniae omp2 proteins, respectively, were compared. To mediate secretion of these proteins the genes were fused to a heterologous signal sequence from murine heavy chain IgG. The nef- and omp2-specific antibody responses were dramatically increased when mice were inoculated with the plasmid encoding the secreted form of these proteins. In contrast, HIV-1 tar comprising an internal strong nuclear targeting sequence could not be induced to secretion and subsequently no enhanced antibody response was observed. Slight improvement of the HIV-1 nef antibody response was achieved after co-inoculation with a granulocyte-macrophage colony stimulating factor (GM-CSF) expression vector. Further, nef-specific T-cell responses were induced after nef DNA injections, and were of Th1-like phenotype regardless of whether the nef protein was secreted or not. The system described in this study, using a plasmid vector with a strong heterologous signal sequence thatmediate efficient antigen secretion in vivo, may have wide applicability for the induction of high antibody levels to normally non-secreted antigens. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:11:27