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Titolo:
Protein interactions and phase behavior: Sensitivity to the form of the pair potential
Autore:
Rosenbaum, DF; Kulkarni, A; Ramakrishnan, S; Zukoski, CF;
Indirizzi:
Univ Illinois, Dept Chem Engn, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 s, Dept Chem Engn, Urbana, IL 61801 USA
Titolo Testata:
JOURNAL OF CHEMICAL PHYSICS
fascicolo: 21, volume: 111, anno: 1999,
pagine: 9882 - 9890
SICI:
0021-9606(199912)111:21<9882:PIAPBS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPERSATURATED LYSOZYME SOLUTIONS; GLOBULAR-PROTEINS; CRYSTAL-GROWTH; CRYSTALLIZATION; SOLUBILITY; SEPARATION; MODEL; TRANSITIONS; DIAGRAM; SULFATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Zukoski, CF Univ Illinois, Dept Chem Engn, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 Engn, Urbana, IL 61801 USA
Citazione:
D.F. Rosenbaum et al., "Protein interactions and phase behavior: Sensitivity to the form of the pair potential", J CHEM PHYS, 111(21), 1999, pp. 9882-9890

Abstract

A robust correlation between experimentally determinable parameter, B-2/B-2(HS), and protein solubility is explored. Here B-2 is the protein solutionsecond virial coefficient and B-2(HS) = 2 pi sigma(3)/3 is the hard spheresecond virial coefficient of the protein whose core is described as a sphere with diameter sigma. The origin of this correlation, which extends over three decades in solubility, is discussed in terms of the phase behavior ofsimple fluids composed of particles experiencing short range attractions. For a given solubility B-2/B-2(HS) values cluster in a narrow range for a variety of protein solution conditions. However, the value of B-2/B-2(HS) atthe metastable fluid/fluid transition remains as sensitive solution conditions. We discuss this sensitivity in terms of phase behavior predicted for particles interacting with the three parameter, square well and Yukawa potentials where metastable critical points are sensitive to the range of attraction. The position of this critical point relative to the solubility boundary and the ease of crystal nucleation are found to depend on solution conditions. (C) 1999 American Institute of Physics. [S0021-9606(99)50644-2].

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Documento generato il 30/09/20 alle ore 08:26:03