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Titolo:
Mode-specific inhibition of sodium-calcium exchange during protein phosphatase blockade
Autore:
Condrescu, M; Hantash, BM; Fang, Y; Reeves, JP;
Indirizzi:
Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Physiol & Pharmacol, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 acol, Newark, NJ 07103 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 47, volume: 274, anno: 1999,
pagine: 33279 - 33286
SICI:
0021-9258(19991119)274:47<33279:MIOSED>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARDIAC NA+-CA2+ EXCHANGER; HAMSTER OVARY CELLS; ATP-DEPENDENT REGULATION; CALYCULIN-A; OKADAIC ACID; NA+/CA2+ EXCHANGE; CA2+-BINDING DOMAIN; NA+,CA2+ EXCHANGE; HUMAN PLATELETS; CA2+;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Reeves, JP Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Physiol & Pharmacol, 185 S Orange Ave, Newark, NJ 07103 USA Univ Med & Dent New Jersey 185 S Orange Ave Newark NJ USA 07103
Citazione:
M. Condrescu et al., "Mode-specific inhibition of sodium-calcium exchange during protein phosphatase blockade", J BIOL CHEM, 274(47), 1999, pp. 33279-33286

Abstract

The effects of the protein phosphatase inhibitors calyculin A and okadaic acid on Na+/Ca2+ exchange activity were examined in transfected Chinese hamster ovary cells expressing the bovine cardiac Na+/Ca2+ exchanger. Incubating the cells for 5-10 min with 100 nM calyculin A reduced exchange-mediatedCa-45(2+) uptake or Ba2+ influx by 50-75%. Half-maximal inhibition of Ca-45(2+) up-take was observed at 15 nM calyculin A. The nonselective protein kinase inhibitors K252a and staurosporine provided partial protection against the effects of calyculin A. Okadaic acid, another protein phosphatase inhibitor, nearly completely blocked exchange-mediated Ba2+ influx. Chinese hamster ovary cells expressing a mutant exchanger in which 420 out of 520 amino acid residues were deleted from the central hydrophilic domain of the exchanger remained sensitive to the inhibitory effects of calyculin A and okadaic acid. Surprisingly, Na-o(+)-dependent Ca2+ efflux appeared to be only modestly inhibited, if at all, by calyculin A or okadaic acid. We conclude that protein hyperphosphorylation during protein phosphatase blockade selectively inhibits the Ca2+ influx mode of Na+/Ca2+ exchange, probably by an indirect mechanism that does not involve phosphorylation of the exchanger itself.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:15:17