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Titolo:
A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes
Autore:
Wang, SY; Cruts, M; Del-Favero, J; Zhang, Y; Tissir, F; Potier, MC; Patterson, D; Nizetic, D; Bosch, A; Chen, HM; Bennett, L; Estivill, X; Kessling, A; Antonarakis, SE; van Broeckhoven, C;
Indirizzi:
Univ Instelling Antwerp, Flanders Inst Biotech, Born Bunge Fdn, Dept Biochem, B-2610 Wilrijk, Belgium Univ Instelling Antwerp Wilrijk Belgium B-2610 , B-2610 Wilrijk, Belgium CNRS, URA, Unite Mixte Rech, Neurobiol Lab, F-2054 Paris, France CNRS Paris France F-2054 Mixte Rech, Neurobiol Lab, F-2054 Paris, France Eleanor Roosevelt Inst Canc Res, Denver, CO 80206 USA Eleanor Roosevelt Inst Canc Res Denver CO USA 80206 Denver, CO 80206 USA Univ London, Sch Pharm, Ctr Appl Mol Biol, London XC1N 1AX, England Univ London London England XC1N 1AX l Mol Biol, London XC1N 1AX, England Hosp Duran & Reynals, Inst Recerca Oncol, Med & Mol Genet Ctr, Barcelona 08907, Catalonia, Spain Hosp Duran & Reynals Barcelona Catalonia Spain 08907 07, Catalonia, Spain Univ Geneva, Sch Med, Div Med Genet, CH-1211 Geneva, Switzerland Univ Geneva Geneva Switzerland CH-1211 enet, CH-1211 Geneva, Switzerland Cantonal Hosp, CH-1211 Geneva, Switzerland Cantonal Hosp Geneva Switzerland CH-1211 sp, CH-1211 Geneva, Switzerland Imperial Coll, Acad Unit Med & Community Genet, Harrow HA1 3UJ, Middx, England Imperial Coll Harrow Middx England HA1 3UJ Harrow HA1 3UJ, Middx, England
Titolo Testata:
GENOME RESEARCH
fascicolo: 11, volume: 9, anno: 1999,
pagine: 1059 - 1073
SICI:
1088-9051(199911)9:11<1059:AHPMOH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-SATELLITE DNA; HUMAN ACROCENTRIC CHROMOSOMES; SOMATIC-CELL HYBRIDS; YAC CONTIG MAP; SEQUENCES; HUMAN-CHROMOSOME-21; MARKERS; CLONES; GENES; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: van Broeckhoven, C Univ Instelling Antwerp, Flanders Inst Biotech, Born Bunge Fdn, Dept Biochem, B-2610 Wilrijk, Belgium Univ Instelling Antwerp Wilrijk Belgium B-2610 elgium
Citazione:
S.Y. Wang et al., "A high-resolution physical map of human chromosome 21p using yeast artificial chromosomes", GENOME RES, 9(11), 1999, pp. 1059-1073

Abstract

The short arm of human chromosome 21 (21p) contains many different types of repetitive sequences and is highly homologous to the short arms of other acrocentric chromosomes. Owing to its repetitive nature and the lack of chromosome 21p-specific molecular markers, most physical maps of chromosome 21exclude this region. We constructed a physical map of chromosome 21p usingsequence tagged site (STS) content mapping of yeast artificial chromosomes(YACs). To this end, 39 STSs located on the short arm or near the centromere of chromosome 21 were constructed, including four polymorphic simple tandem repeats (STRs) and two expressed sequence tags (ESTs). Thirty YACs wereselected From the St. Louis YAC library, the chromosome 21-enriched ICRF YAC library, and the CEPH YAC and megaYAC libraries. These were assembled ina YAC contig map ranging from the centromere to the rDNA gene cluster at 21p12. The total size of the region covered by YACs is estimated between 2.9and 5 Mb. The integrity of the YAC contig was confirmed by restriction enzyme fingerprinting and fluorescence in situ hybridization (FISH). One gap with an estimated size of 400 kb remained near the telomeric end of the contig. This YAC contig map of the short arm of human chromosome 21 constitutesa basic framework for Further structural and functional studies of chromosome 21p.

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Documento generato il 18/09/20 alle ore 10:16:55