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Titolo:
Vasopressin stimulates long-term net chloride secretion in cortical collecting duct cells
Autore:
Djelidi, S; Fay, M; Cluzeaud, F; Thomas-Soumarmon, A; Bonvalet, JP; Farman, N; Blot-Chabaud, M;
Indirizzi:
Fac Med Xavier Bichat, Inst Fed Rech Cellules Epitheliales, INSERM, U478, Paris, France Fac Med Xavier Bichat Paris France eliales, INSERM, U478, Paris, France Fac Med Xavier Bichat, Inst Fed Rech Cellules Epitheliales, INSERM, U10, Paris, France Fac Med Xavier Bichat Paris France heliales, INSERM, U10, Paris, France
Titolo Testata:
FEBS LETTERS
fascicolo: 3, volume: 460, anno: 1999,
pagine: 533 - 538
SICI:
0014-5793(19991105)460:3<533:VSLNCS>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSMEMBRANE CONDUCTANCE REGULATOR; ARGININE-VASOPRESSIN; NA+ REABSORPTION; CL SECRETION; RAT-KIDNEY; APICAL CL; A6 CELLS; FIBROSIS; EXPRESSION; GENE;
Keywords:
cystic fibrosis transmembrane regulator; Na+/K+/2Cl(-); vasopressin; kidney; cortical collecting duct;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Blot-Chabaud, M Fac Med Xavier Bichat, Inst Fed Rech Cellules Epitheliales, INSERM, U478, Paris, France Fac Med Xavier Bichat Paris France U478, Paris, France
Citazione:
S. Djelidi et al., "Vasopressin stimulates long-term net chloride secretion in cortical collecting duct cells", FEBS LETTER, 460(3), 1999, pp. 533-538

Abstract

The classical short-term effect (within minutes) of arginine vasopressin (AVP) consists in increasing sodium, chloride and water transport in kidney cells, More recently, long-term actions (several hours) of the hormone havebeen evidenced on water and sodium fluxes, due to transcriptional enhancement in the expression of their transporters. The present study demonstratesthat AVP is also responsible for a long-term increase in net chloride secretion. In the RCCD1 rat cortical collecting duct cell line, 10(-8) M AVP induced, after several hours, an increase in net Cl-36(-) secretion. This delayed effect of AVP was inhibited by basal addition of 10(-4) M bumetanide and apical addition of 10(-4) RI glibenclamide, suggesting chloride entry atthe basal membrane through a Na+/K+/2Cl(-) and apical secretion through a chloride conductance. An original acute cell permeabilization method was developed to allow for entry of antibodies directed against the regulatory region (R) of the cystic fibrosis transmembrane regulator (CFTR) into the cells, This procedure led to a complete and specific blocking of the longterm net chloride secretion induced by AVP, Finally, it was observed that CFTR transcripts steady-state level was significantly increased by AVP treatment. Besides the well-documented short-term effect of AVP on chloride transport, these results provide evidence that in RCCD1 cells, AVP induces a delayedincrease in transepithelial net chloride secretion that is mediated by a Na+/K+/2Cl(-) co-transporter and CFTR, (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:12:24