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Titolo:
Spinach hexokinase I is located in the outer envelope membrane of plastids
Autore:
Wiese, A; Groner, F; Sonnewald, U; Deppner, H; Lerchl, J; Hebbeker, U; Flugge, UI; Weber, A;
Indirizzi:
Univ Cologne, Lehrstuhl Bot 2, D-50931 Cologne, Germany Univ Cologne Cologne Germany D-50931 uhl Bot 2, D-50931 Cologne, Germany Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany Inst Pflanzengenet & Kulturpflanzenforsch Gatersleben Germany D-06466 ny
Titolo Testata:
FEBS LETTERS
fascicolo: 1-2, volume: 461, anno: 1999,
pagine: 13 - 18
SICI:
0014-5793(19991112)461:1-2<13:SHIILI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHATE TRANSLOCATOR ACTIVITY; SACCHAROMYCES-CEREVISIAE; ENDOPLASMIC-RETICULUM; MOLECULAR-CLONING; GLUCOSE-TRANSPORT; SINK-REGULATION; CHLOROPLASTS; PROTEIN; IMPORT; LEAVES;
Keywords:
hexokinase; plant; glucose transport; plastid envelope membrane; sugar signalling; subcellular localization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Weber, A Univ Cologne, Lehrstuhl Bot 2, Gyrhofstr 15, D-50931 Cologne, Germany Univ Cologne Gyrhofstr 15 Cologne Germany D-50931 logne, Germany
Citazione:
A. Wiese et al., "Spinach hexokinase I is located in the outer envelope membrane of plastids", FEBS LETTER, 461(1-2), 1999, pp. 13-18

Abstract

The subcellular localization of hexokinase activities in plant cells has been a matter of debate for a long time. We have isolated a hexokinase cDNA fragment from glucose-fed spinach leaves using a differential display reverse transcription-PCR approach. The corresponding cDNA was expressed in Escherichia coli and an antiserum, raised against the recombinant protein, was used in subcellular localization studies. The spinach hexokinase could be localized primarily to the outer envelope membrane of chloroplasts where it is inserted via its N-terminal membrane anchor. We suggest that the chloroplast envelope hexokinase is involved in the energization of glucose export from plastids rather than in the sugar-sensing pathway of the plant cell. (C) 1999 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 22:54:00