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Titolo:
Androgen receptor mRNA expression in the rhesus monkey ovary
Autore:
Duffy, DM; Abdelgadir, SE; Stott, KR; Resko, JA; Stouffer, RL; Zelinski-Wooten, MB;
Indirizzi:
Oregon Reg Primate Res Ctr, Div Reprod Sci, Beaverton, OR 97006 USA OregonReg Primate Res Ctr Beaverton OR USA 97006 Beaverton, OR 97006 USA Oregon Hlth Sci Univ, Dept Physiol & Pharmacol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 rmacol, Portland, OR 97201 USA
Titolo Testata:
ENDOCRINE
fascicolo: 1, volume: 11, anno: 1999,
pagine: 23 - 30
SICI:
1355-008X(199908)11:1<23:ARMEIT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMATE CORPUS-LUTEUM; MENSTRUAL-CYCLE; GRANULOSA-CELLS; DEVELOPMENTAL REGULATION; CHORIONIC-GONADOTROPIN; INVITRO FERTILIZATION; STEROIDOGENIC ENZYMES; GENE-EXPRESSION; RAT OVARY; PROGESTERONE;
Keywords:
androgen receptor; ovary; primate; menstrual cycle; corpus luteum; granulosa cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Duffy, DM Oregon Reg Primate Res Ctr, Div Reprod Sci, 505 NW 185th Ave, Beaverton, OR 97006 USA Oregon Reg Primate Res Ctr 505 NW 185th Ave BeavertonOR USA 97006
Citazione:
D.M. Duffy et al., "Androgen receptor mRNA expression in the rhesus monkey ovary", ENDOCRINE, 11(1), 1999, pp. 23-30

Abstract

Immunocytochemical detection of androgen receptors (ARs) in several compartments of the macaque ovary, including the germinal epithelium, follicle, and corpus luteum, suggests a role for androgens in modulating ovarian function via the classical receptor-mediated pathway. To examine AR mRNA expression in the rhesus monkey ovary, total RNA was isolated from whole ovaries, the germinal epithelium-enriched cortical and medullary compartments of theovary, and corpora lutea from early (d 3-5), mid (d 6-8), mid-late (d 10-12), and late (d 13-15) stages of the luteal phase of the menstrual cycle. RNA was also obtained from luteinized granulosa cells from monkeys receivinggonadotropin treatment to stimulate the development of multiple ovarian follicles. After reverse transcription of total RNA using oligo-dT as a primer, polymerase chain reaction (PCR) was used to amplify a unique 329 bp segment of the monkey AR hormone-binding region. Reverse transcriptase (RT)-PCRproducts of the expected size were detected in all ovarian and control tissues. Sequence analysis of the AR cDNA from the macaque ovary revealed 99% nucleotide homology and 100% predicted amino acid homology to the cDNA for the hormone-binding region of human AR. Northern analysis demonstrated the presence of a major AR mRNA species at 9.5 kb in corpus luteum, luteinized granulosa cells, and prostate, with additional bands detected in the corpusluteum and prostate at 7.9 and 3.4 kb, respectively. A sensitive RNase protection assay was used to examine AR mRNA levels in ovarian tissues and showed AR mRNA expression throughout the life-span of the corpus luteum. Thus,detection of AR mRNA in the primate ovary, including the periovulatory follicle and corpus luteum, supports the concept that these tissues are targets for receptor-mediated androgen action during the menstrual cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 09:33:22