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Titolo:
Immunolocalization of Na+/K+-ATPase in mitochondrion-rich cells of the atlantic hagfish (Myxine glutinosa) gill
Autore:
Choe, KP; Edwards, S; Morrison-Shetlar, AI; Toop, T; Claiborne, JB;
Indirizzi:
Georgia So Univ, Dept Biol, Statesboro, GA 30460 USA Georgia So Univ Statesboro GA USA 30460 pt Biol, Statesboro, GA 30460 USA Deakin Univ, Dept Chem & Biol Sci, Geelong, Vic 3217, Australia Deakin Univ Geelong Vic Australia 3217 Sci, Geelong, Vic 3217, Australia Mt Desert Isl Biol Lab, Salsbury Cove, ME 04672 USA Mt Desert Isl Biol Lab Salsbury Cove ME USA 04672 bury Cove, ME 04672 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 2, volume: 124, anno: 1999,
pagine: 161 - 168
SICI:
1095-6433(199910)124:2<161:IONIMC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRESH-WATER; CHLORIDE CELLS; MARINE TELEOST; EPITHELIUM; MEMBRANE; SEAWATER;
Keywords:
acid-base regulation; agnatha; craniate; evolution; ion regulation; ATPase; vertebrate; western blot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Choe, KP Univ Florida, Dept Zool, 223 Bartram Hall,Box 118525, Gainesville, FL 32611 USA Univ Florida 223 Bartram Hall,Box 118525 Gainesville FL USA 32611
Citazione:
K.P. Choe et al., "Immunolocalization of Na+/K+-ATPase in mitochondrion-rich cells of the atlantic hagfish (Myxine glutinosa) gill", COMP BIOC A, 124(2), 1999, pp. 161-168

Abstract

Using a monoclonal antibody for the a-subunit of the Na+/K+-ATPase, DASPEI(a vital mitochondria dye), and confocal laser scanning microscopy, the presence of Na+/K+-ATPase in mitochondrion-rich cells of the hagfish gill wasconfirmed. In addition, the level of Na+/K+-ATPase expression in the hagfish gill was compared to that of fishes with different osmoregulatory strategies (little skate, Raja erinacea and mummichog, Fundulus heteroclitus). Immunocytochemistry detected a high density of columnar cells expressing Na+/K+-ATPase in the afferent filamental epithelium. Positive cells were also found in the lamellar epithelium but at a much lower density. The distribution of DASPEI staining was similar to that of the Na+/K+-ATPase antibody, indicating that the enzyme is expressed in mitochondrion-rich cells. Immunoblot analysis confirmed the specificity of the antibody for the 97 kDa alpha-subunit of the enzyme. The immunoreactive band intensity for the Atlantic hagfish was similar to that of the little skate, but less than half that of the full-strength seawater mummichog. These results are discussed in relation to gill function in early craniates. (C) 1999 Elsevier Science Inc. All rights reserved.

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Documento generato il 05/12/20 alle ore 01:30:02