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Titolo:
In vitro metabolic and respiratory acidosis selectively inhibit osteoblastic matrix gene expression
Autore:
Frick, KK; Bushinsky, DA;
Indirizzi:
Univ Rochester, Sch Med, Dept Med, Nephrol Unit, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 phrol Unit, Rochester, NY 14642 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 5, volume: 277, anno: 1999,
pagine: F750 - F755
SICI:
0363-6127(199911)277:5<F750:IVMARA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
URINARY CALCIUM EXCRETION; SURFACE ION COMPOSITION; NODULES FORMED INVITRO; MESSENGER-RNA; BONE CALCIUM; ALKALINE-PHOSPHATASE; GLA PROTEIN; IN-VITRO; PH; MINERALIZATION;
Keywords:
bone; matrix Gla protein; osteonectin; osteopontin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Frick, KK Univ Rochester, Sch Med, Dept Med, Nephrol Unit, 601 Elmwood Ave,Box 675, Rochester, NY 14642 USA Univ Rochester 601 Elmwood Ave,Box 675 Rochester NY USA 14642 SA
Citazione:
K.K. Frick e D.A. Bushinsky, "In vitro metabolic and respiratory acidosis selectively inhibit osteoblastic matrix gene expression", AM J P-REN, 277(5), 1999, pp. F750-F755

Abstract

Clinically, a decrease in blood pH may be due to either a reduction in bicarbonate concentration ([HCO3-], metabolic acidosis) or an increase in Pco(2) (respiratory acidosis). In mammals, metabolic acidosis induces a far greater increase in urine calcium excretion than respiratory acidosis. In cultured bone, metabolic acidosis induces a marked increase in calcium efflux and a decrease in osteoblastic collagen synthesis, whereas isohydric respiratory acidosis has little effect on either parameter. We have shown that metabolic acidosis prevents the normal developmental increase in the expression of RNA for matrix Gla protein and osteopontin in chronic cultures of primary murine calvarial bone cells (predominantly osteoblasts) but does not alter expression of osteonectin. To compare the effects of isohydric metabolic and respiratory acidosis on expression of these genes, bone cell cultureswere incubated in medium at pH similar to 7.2 to model metabolic ([HCO3-],similar to 13 mM) or respiratory (Pco(2), similar to 80 mmHg) acidosis or at pH similar to 7.4 as a control. Cells were sampled at weeks 4, 5, and 6 to assess specific RNA content. At all time periods studied, both metabolicand respiratory acidosis inhibited the expression of RNA for matrix Gla protein and osteopontin to a similar extent, whereas there was no change in osteonectin expression. In contrast to the significant difference in the effects of metabolic and respiratory acidosis on bone calcium efflux and osteoblastic collagen synthesis, these two forms of acidosis have a similar effect on osteoblastic RNA expression of both matrix Gla protein and osteopontin. Thus, although several aspects of bone cell function are dependent on the type of acidosis, expression of these two matrix genes appears to be regulated by extracellular pH, independently of the type of acidosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:25:49