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Titolo:
Expression of voltage-dependent K+ channel genes in mesenteric artery smooth muscle cells
Autore:
Xu, CL; Lu, YY; Tang, GH; Wang, R;
Indirizzi:
Univ Saskatchewan, Coll Med, Dept Physiol, Saskatoon, SK S7N 5E5, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5E5 skatoon, SK S7N 5E5, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 5, volume: 277, anno: 1999,
pagine: G1055 - G1063
SICI:
0193-1857(199911)277:5<G1055:EOVKCG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT PULMONARY; SUBUNITS; CURRENTS; KV2.1; MYOCYTES; KV1.5; IDENTIFICATION; LOCALIZATIONS; CLONING; MEMBERS;
Keywords:
Kv channels; peripheral artery; reverse transcription-polymerase chain reaction; Western blot; immunocytochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, R Univ Saskatchewan, Coll Med, Dept Physiol, Saskatoon, SK S7N 5E5, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5E5 SK S7N 5E5, Canada
Citazione:
C.L. Xu et al., "Expression of voltage-dependent K+ channel genes in mesenteric artery smooth muscle cells", AM J P-GAST, 277(5), 1999, pp. G1055-G1063

Abstract

Molecular basis of native voltage-dependent K+ (Kv) channels in smooth muscle cells (SMCs) from rat mesenteric arteries was investigated. The whole cell patch-clamp study revealed that a 4-aminopyridine-sensitive delayed rectifier K+ current (I-K) was the predominant K+ conductance in these cells. A systematic screening of the expression of 18 Kv channel genes using RT-PCR technique showed that six I-K-encoding genes (Kv1.2, Kv1.3, Kv1.5, Kv2.1,Kv2.2, and Kv3.2) were expressed in mesenteric artery. Although no transient outward Kv currents (I-A) were recorded in the studied SMCs, transcriptsof multiple I-A-encoding genes, including Kv1.4, Kv3.3, Kv3.4, Kv4.1, Kv4.2, and Kv4.3 as well as I-A-facilitating Kv beta-subunits (Kv beta 1, Kv beta 2, and Kv beta 3), were detected in mesenteric arteries. Western blot analysis demonstrated that four I-K-related Kv channel proteins (Kv1.2, Kv1.3, Kv1.5, and Kv2.1) were detected in mesenteric artery tissues. The presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 channel proteins in isolated SMCs was further confirmed by immunocytochemistry study. Our results suggest that thenative I-K in rat mesenteric artery SMCs might be generated by heteromultimerization of Kv genes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:49:48