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Titolo:
Fluorescent oligonucleotides - Versatile tools as probes and primers for DNA and RNA analysis
Autore:
Wojczewski, C; Stolze, K; Engels, JW;
Indirizzi:
Univ Frankfurt, Inst Organ Chem, D-60439 Frankfurt, Germany Univ Frankfurt Frankfurt Germany D-60439 hem, D-60439 Frankfurt, Germany Humboldt Univ, Inst Organ & Bioorgan Chem, D-10115 Berlin, Germany Humboldt Univ Berlin Germany D-10115 organ Chem, D-10115 Berlin, Germany
Titolo Testata:
SYNLETT
fascicolo: 10, , anno: 1999,
pagine: 1667 - 1678
SICI:
0936-5214(199910):10<1667:FO-VTA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE SYNTHESIS; ENERGY-TRANSFER; LABELED OLIGONUCLEOTIDES; THERMAL-STABILITY; MULTIPLE FLUOROPHORES; SENSITIVE DETECTION; SEQUENCE ANALYSIS; ESCHERICHIA-COLI; NUCLEIC-ACIDS; BASE-PAIRS;
Keywords:
fluorescent oligonucleotides; automated solid-phase synthesis; modified nucleosides; fluorescence spectroscopy; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
97
Recensione:
Indirizzi per estratti:
Indirizzo: Engels, JW Univ Frankfurt, Inst Organ Chem, Marie Curie Str 11, D-60439 Frankfurt, Germany Univ Frankfurt Marie Curie Str 11 Frankfurt Germany D-60439 ny
Citazione:
C. Wojczewski et al., "Fluorescent oligonucleotides - Versatile tools as probes and primers for DNA and RNA analysis", SYNLETT, (10), 1999, pp. 1667-1678

Abstract

Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:26:21