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Titolo:
Improvement of lymphocyte proliferation in human immunodeficiency virus infection after recombinant interleukin-2 treatment
Autore:
Afzelius, P; Nielsen, SD; Nielsen, JO; Hansen, JES;
Indirizzi:
Univ Copenhagen, Hvidovre Hosp, Dept Infect Dis 144, DK-2670 Hvidovre, Denmark Univ Copenhagen Hvidovre Denmark DK-2670 144, DK-2670 Hvidovre, Denmark
Titolo Testata:
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES
fascicolo: 5, volume: 31, anno: 1999,
pagine: 437 - 442
SICI:
0036-5548(1999)31:5<437:IOLPIH>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; POKEWEED MITOGEN; HIV-1 INFECTION; T-LYMPHOCYTES; EXPRESSION; ACTIVATION; INDIVIDUALS; ANTIBODIES; GROWTH; INCREASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Afzelius, P Univ Copenhagen, Hvidovre Hosp, Dept Infect Dis 144, KettegardAlle 30, DK-2670 Hvidovre, Denmark Univ Copenhagen Kettegard Alle 30 Hvidovre Denmark DK-2670 rk
Citazione:
P. Afzelius et al., "Improvement of lymphocyte proliferation in human immunodeficiency virus infection after recombinant interleukin-2 treatment", SC J IN DIS, 31(5), 1999, pp. 437-442

Abstract

In this study, the effect of recombinant interleukin-2 (rIL-2) on the function of peripheral blood mononuclear cells (PBMC) from human immunodeficiency virus (HIV)-infected patients was examined. Using polymerase chain reaction (PCR), an impaired ability of PBMC from 8 patients to respond upon mitogen stimulation with expression of IL-2 and IL-2 receptor (IL-2R) messengerribonucleic acid (mRNA) was found compared with healthy donors (p = 0.02 and p = 0.05, respectively), Flow cytometry was used to determine the expression of p55 interleukin-2 a-receptor (CD25) after phytohaemagglutinin (PHA)-stimulation. Induced CD25 expression in response to stimulation aas lower in patient cells than in donor cells (in CD4(+) (p = 0.01) and in CD8(+) (p= 0.03)), After rIL-2 treatment, the functionality of ex vivo expanded PBMC from patients was restored to the level found in donors. Finally, the induced gene expressions for IL-2 and IL-2R were positively correlated (p < 0.0001), suggesting that the activation of the IL-2 and IL-2R genes in humansmay share a common activation pathway, as has been found in monkeys infected by simian immunodeficiency virus (SIV). These results indicate the existence of a reversible IL-2 and IL-2R defect at the pretranscriptional or transcriptional level in PBMC from patients. This may help explain the T-cell anergy found during HIV-infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:01:04