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Titolo:
The maize EmBP-1 orthologue differentially regulates Opaque2-dependent gene expression in yeast and cultured maize endosperm cells
Autore:
Carlini, LE; Ketudat, M; Parsons, RL; Prabhakar, S; Schmidt, RJ; Guiltinan, MJ;
Indirizzi:
Penn State Univ, Dept Hort, Wartik Lab 302, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 2, University Pk, PA 16802 USA Univ Calif San Diego, Dept Biol, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t Biol, La Jolla, CA 92093 USA Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 Genet, La Jolla, CA 92093 USA
Titolo Testata:
PLANT MOLECULAR BIOLOGY
fascicolo: 3, volume: 41, anno: 1999,
pagine: 339 - 349
SICI:
0167-4412(199910)41:3<339:TMEODR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING-SPECIFICITY; LEUCINE ZIPPER PROTEIN; G-BOX; TRANSCRIPTIONAL REGULATION; NUCLEOTIDE-SEQUENCES; OPAQUE-2 MUTATION; BZIP PROTEINS; ELEMENT; FAMILY; TRANSACTIVATION;
Keywords:
bZIP; dimerization; EmBP-1; G-box; Opaque2; zein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Guiltinan, MJ Penn State Univ, Dept Hort, Wartik Lab 302, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 Pk, PA 16802 USA
Citazione:
L.E. Carlini et al., "The maize EmBP-1 orthologue differentially regulates Opaque2-dependent gene expression in yeast and cultured maize endosperm cells", PLANT MOL B, 41(3), 1999, pp. 339-349

Abstract

In addition to the bZIP protein Opaque2 (O2), there are other maize endosperm nuclear proteins that recognize the O2 box in 22 kDa zein gene promoters. In an effort to understand the effect of these factors on 22 kDa zein expression, we have cloned one of these and identified it as the putative maize (Zea mays L.) orthologue of the wheat bZIP protein EmBP-1 (mEmBP-1). ThemEmBP-1 protein exhibits 52% sequence identity and 68% similarity with thewheat protein and recognizes a similar spectrum of DNA sequences, albeit with slightly altered specificity. The mEmBP-1 gene exists as duplicate lociin maize on chromosomes 7S (mEmBP-1a) and 2L (mEmBP-1b). The mEmBP-1 genesare expressed in endosperm, embryo, immature ears, tassel, roots, and seedling shoots at low levels. Although mEmBP-1 binds to the O2 box from the 22kDa zein gene promoter as a homodimer, it is unable to heterodimerize withO2. The mEmBP-1 protein can activate transcription from a truncated promoter containing a pentamer of the O2 site in yeast cells; however, it inhibited regulated transcription of a 22 kDa zein promoter in a transient expression assay using cultured maize endosperm cells.

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Documento generato il 04/12/20 alle ore 09:19:50