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Titolo:
The nitric oxide pathway in pig isolated calyceal smooth muscle
Autore:
Iselin, CE; Ny, L; Mastrangelo, D; Felley-Bosco, E; Larsson, B; Alm, P; Andersson, KE;
Indirizzi:
Univ Hosp Geneva, Dept Surg, Urol Clin, CH-1211 Geneva 14, Switzerland Univ Hosp Geneva Geneva Switzerland 14 n, CH-1211 Geneva 14, Switzerland
Titolo Testata:
NEUROUROLOGY AND URODYNAMICS
fascicolo: 6, volume: 18, anno: 1999,
pagine: 673 - 685
SICI:
0733-2467(1999)18:6<673:TNOPIP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL RENAL PELVIS; LOWER URINARY-TRACT; GUINEA-PIG; NONCHOLINERGIC RELAXATION; CARBON-MONOXIDE; HUMAN URETER; SYNTHASE; NERVES; LOCALIZATION; CONTRACTILE;
Keywords:
nitric oxide; smooth muscle; renal calyces; kidney pelvis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Iselin, CE Univ Hosp Geneva, Dept Surg, Urol Clin, CH-1211 Geneva 14, Switzerland Univ Hosp Geneva Geneva Switzerland 14 Geneva 14, Switzerland
Citazione:
C.E. Iselin et al., "The nitric oxide pathway in pig isolated calyceal smooth muscle", NEUROUROL U, 18(6), 1999, pp. 673-685

Abstract

In pig and humans, whose kidneys have a multi-calyceal collecting system, the initiation of ureteral peristalsis takes place in the renal calyces. Inthe pig and human ureter, recent evidence suggests that nitric bride (NO) is an inhibitory mediator that may be involved in the regulation of peristalsis. This study was designed to assess whether the NO synthase/NO/cyclic GMP pathway modulates the motility of pig isolated calyceal smooth muscle. Immunohistochemistry revealed a moderate overall innervation of the smooth muscle layer, and no neuronal or inducible NO synthase (NOS) immunoreactivities. Endothelial NOS immunoreactivities were observed in the urothelium andvascular endothelium, and numerous cyclic GMP-immunoreactive (-IR) calyceal smooth muscle cells were found. As measured by monitoring the conversion of L-arginine to L-citrulline, Ca2+-dependent NOS activity was moderate. Assessment of functional effects was performed in tissue baths and showed that NO and SIN-1 decreased spontaneous and induced contractions of isolated preparations in a concentration-dependent manner. In strips exposed to NO, there was a 10-fold increase of the cyclic GMP levels compared with control preparations (P < 0.01). It is concluded that a non-neuronal NOS/NO/cyclic GMP pathway is present in pig calyces, where it may influence motility. Thedemonstration of cyclic GMP-IR smooth muscle cells suggests that NO acts directly on these cells. This NOS/NO/cyclic GMP pathway may be a target for drugs inhibiting peristalsis of mammalian upper urinary tract. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 15:00:23