Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The murine clan VHIII related 7183, J606 and S107 and DNA4 families commonly encode for binding to a bacterial B cell superantigen
Autore:
Cary, S; Krishnan, M; Marion, TN; Silverman, GJ;
Indirizzi:
Univ Calif San Diego, Sam & Rose Stein Inst Res Aging, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 Aging, La Jolla, CA 92093 USA Univ Calif San Diego, Dept Med, Theodore Gildred Canc Ctr, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 nc Ctr, La Jolla, CA 92093 USA Univ Tennessee, Dept Microbiol & Immunol, Memphis, TN 38163 USA Univ Tennessee Memphis TN USA 38163 biol & Immunol, Memphis, TN 38163 USA
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 11-12, volume: 36, anno: 1999,
pagine: 769 - 776
SICI:
0161-5890(199908)36:11-12<769:TMCVR7>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
STAPHYLOCOCCAL PROTEIN-A; RECEPTOR-BETA-CHAIN; GENE USAGE; IMMUNOGLOBULIN-BINDING; CRYSTAL-STRUCTURE; SOMATIC MUTATION; VARIABLE REGION; FAB FRAGMENT; HUMAN-IGM; ANTIBODIES;
Keywords:
repertoire development; superantigens; B lymphocyte; variable region;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Silverman, GJ Univ Calif San Diego, Sam & Rose Stein Inst Res Aging, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 la, CA 92093 USA
Citazione:
S. Cary et al., "The murine clan VHIII related 7183, J606 and S107 and DNA4 families commonly encode for binding to a bacterial B cell superantigen", MOL IMMUNOL, 36(11-12), 1999, pp. 769-776

Abstract

Superantigens, by virtue of their unconventional binding interactions withAg receptors, can simulate a large subset of mature lymphocytes in the repertoire. Recent studies have documented that in vivo exposure to the model bacterial B cell superantigen, Staphylococcal protein A (SpA), induces large scale effects on murine B-cell clonal selection by mechanism(s) that include deletion of supra-clonal sets. While the structural bases for the immunomodulatory properties of several T-cell superantigens have been well characterized, the requirements for murine Fab-binding of SpA remain incompletely defined. To investigate these structural requirements, a series of directbinding and inhibition studies were performed with a large panel of Moabs of diverse variable region gene usage. These studies confirm previous reports that superantigen binding is completely restricted to the products of dan V-H III-related families, that include the small S107 and J606 families, and we also demonstrated that usage of the related small DNA4 family commonly correlates with weaker binding activity. Furthermore, our results document that genes from the largest dan V-H III family, 7183, commonly encode for Fab-mediated binding of SpA, while antibodies from five other VH families. J558, Q52, Sm7, VH11 and VH12, did not display Fab-mediated SpA binding activity. By contributing to the essential foundation for understanding of the structural basis for binding interactions, these findings will aid interpretation of evolving observations regarding the clonal fates induced by invivo B-cell superantigen exposure. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:09:34