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Titolo:
Characterization of an in vitro model off elastic fiber assembly
Autore:
Robb, BW; Wachi, H; Schaub, T; Mecham, RP; Davis, EC;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Cell Biol & Neurosci, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Cell Biol & Neurosci, Dallas, TX 75235 USA Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ St Louis MO USA 63110 l & Physiol, St Louis, MO 63110 USA Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 d, Dept Med, St Louis, MO 63110 USA
Titolo Testata:
MOLECULAR BIOLOGY OF THE CELL
fascicolo: 11, volume: 10, anno: 1999,
pagine: 3595 - 3605
SICI:
1059-1524(199911)10:11<3595:COAIVM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SUPRAVALVULAR AORTIC-STENOSIS; DEVELOPING MOUSE AORTA; MUSCLE CELL-CULTURES; EXTRACELLULAR-MATRIX; EPITHELIAL-CELLS; POINT MUTATIONS; MARFAN-SYNDROME; GENE; TROPOELASTIN; DEGRADATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Davis, EC Univ Texas, SW Med Ctr, Dept Cell Biol & Neurosci, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 & Neurosci, Dallas, TX 75235 USA
Citazione:
B.W. Robb et al., "Characterization of an in vitro model off elastic fiber assembly", MOL BIOL CE, 10(11), 1999, pp. 3595-3605

Abstract

Elastic fibers consist of two morphologically distinct components: elastinand 10-nm fibrillin-containing microfibrils. During development, the microfibrils form bundles that appear to act as a scaffold for the deposition, orientation, and assembly of tropoelastin monomers into an insoluble elasticfiber. Although microfibrils can assemble independent of elastin, tropoelastin monomers do not assemble without the presence of microfibrils. In the present study, immortalized ciliary body pigmented epithelial (PE) cells were investigated for their potential to serve as a cell culture model for elastic fiber assembly. Northern analysis showed that the PE cells express microfibril proteins but do not express tropoelastin. Immunofluorescence staining and electron microscopy confirmed that the microfibril proteins produced by the PE cells assemble into intact microfibrils. When the PE cells were transfected with a mammalian expression vector containing a bovine tropoelastin cDNA, the cells were found to express and secrete tropoelastin. Immunofluorescence and electron microscopic examination of the transfected PE cells showed the presence of elastic fibers in the matrix. Biochemical analysis of this matrix showed the presence of cross-links that are unique to mature insoluble elastin. Together, these results indicate that the PE cells provide a unique, stable in vitro system in which to study elastic fiber assembly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:14:00