Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Adapting a compact confocal microscope system to a two-photon excitation fluorescence imaging architecture
Autore:
Diaspro, A; Corosu, M; Ramoino, P; Robello, M;
Indirizzi:
Univ Genoa, Dept Phys, I-16146 Genoa, Italy Univ Genoa Genoa Italy I-16146 iv Genoa, Dept Phys, I-16146 Genoa, Italy Univ Genoa, INFM, I-16146 Genoa, Italy Univ Genoa Genoa Italy I-16146Univ Genoa, INFM, I-16146 Genoa, Italy Univ Genoa, DIPTERIS, I-16124 Genoa, Italy Univ Genoa Genoa Italy I-16124 niv Genoa, DIPTERIS, I-16124 Genoa, Italy
Titolo Testata:
MICROSCOPY RESEARCH AND TECHNIQUE
fascicolo: 3, volume: 47, anno: 1999,
pagine: 196 - 205
SICI:
1059-910X(19991101)47:3<196:AACCMS>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
2-PHOTON EXCITATION; SECTIONS; IMAGES; NM;
Keywords:
two-photon microscopy; single-pinhole confocal microscopy; three-dimensional point-spread function; helical biostructure; 3D imaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Diaspro, A Univ Genoa, Dept Phys, Via Dodecaneso 33, I-16146 Genoa, Italy Univ Genoa Via Dodecaneso 33 Genoa Italy I-16146 Genoa, Italy
Citazione:
A. Diaspro et al., "Adapting a compact confocal microscope system to a two-photon excitation fluorescence imaging architecture", MICROSC RES, 47(3), 1999, pp. 196-205

Abstract

Within the framework of a national National Institute of Physics of Matter(INFM) project, we have realised a two-photon excitation (TPE) fluorescence microscope based on a new generation commercial confocal scanning head. The core of the architecture is a mode-locked Ti:Sapphire laser (Tsunami 3960, Spectra Physics Inc., Mountain View, CA) pumped by a high-power (5 W, 532 nm) laser (Millennia V, Spectra Physics Inc.) and an ultracompact confocal scanning head, Nikon PCM2000 (Nikon Instruments, Florence, Italy) using asingle-pinhole design. Three-dimensional point-spread function has been measured to define spatial resolution performances. The TPE microscope has been used with a wide range of excitable fluorescent; molecules (DAPI, Fura-2, Indo-1, DiOC(6)(3), fluoresceine, Texas red) covering a single photon spectral range from W to green. An example is reported on 3D imaging of the helical structure of the sperm head of the Octopus Eledone cirrhosa labelled with an UV excitable dye, i.e., DAPI. The system can be easily switched foroperating both in conventional and two-photon mode. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 05:03:57