Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Solubilization and stabilization of carotenoids using micelles: Delivery of lycopene to cells in culture
Autore:
Xu, XY; Wang, Y; Constantinou, AI; Stacewicz-Sapuntzakis, M; Bowen, PE; van Breemen, RB;
Indirizzi:
Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 & Pharmacognosy, Chicago, IL 60612 USA Univ Illinois, Dept Surg Oncol, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Dept Surg Oncol, Chicago, IL 60612 USA Univ Illinois, Dept Human Nutr & Dietet, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 n Nutr & Dietet, Chicago, IL 60612 USA
Titolo Testata:
LIPIDS
fascicolo: 10, volume: 34, anno: 1999,
pagine: 1031 - 1036
SICI:
0024-4201(199910)34:10<1031:SASOCU>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-CAROTENE; ABSORPTION; PROSTATE; RETINOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: van Breemen, RB Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy, 833 S Wood St, Chicago, IL 60612 USA Univ Illinois 833 S Wood St Chicago ILUSA 60612 60612 USA
Citazione:
X.Y. Xu et al., "Solubilization and stabilization of carotenoids using micelles: Delivery of lycopene to cells in culture", LIPIDS, 34(10), 1999, pp. 1031-1036

Abstract

The use of the organic cosolvents tetrahydrofuran and dimethylsulfoxide was found to be unsuitable for prostate tumor cell cultures because of solvent cytotoxicity and the poor solubility and instability of lycopene. For example, the half-life of lycopene in organic/aqueous solution was found to beless than 2 h. Therefore, a micellar preparation of lycopene was developedfor the solubilization and stabilization of lycopene in cell culture media. Neither the micelles themselves nor lycopene solubilized in micelles at concentrations up to 10 mu g/mL in the cell culture media produced cytotoxicity or inhibition of cell proliferation in either LNCaP human prostate cells or Hs888Lu human lung cells. Lycopene solubilized in micelles was stable for at least 96 h under standard cell culture conditions so that a constantlycopene supply could be provided to the cells. During the culture process, lycopene was taken up by LNCaP cells and reached a plateau at approximately 12 h. Micelles provide a convenient, inexpensive, and nontoxic vehicle for dissolving and stabilizing carotenes such as lycopene in tissue culture media and then delivering them to cells growing in culture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:16:26