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Titolo:
Neovascularization during venous thrombosis organization: A preliminary study
Autore:
Wakefield, TW; Linn, MJ; Henke, PK; Kadell, AM; Wilke, CA; Wrobleski, SK; Sarkar, M; Burdick, MD; Myers, DD; Strieter, RM;
Indirizzi:
Univ Michigan, Med Ctr, Dept Surg,Jobst Vasc Surg Lab, Vasc Surg Sect,Taubman Hlth Care Ctr 2210, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MIUSA 48109 re Ctr 2210, Ann Arbor, MI 48109 USA Univ Michigan, Med Ctr, Dept Med, Div Pulm & Crit Care Med, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 it Care Med, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF VASCULAR SURGERY
fascicolo: 5, volume: 30, anno: 1999,
pagine: 885 - 892
SICI:
0741-5214(199911)30:5<885:NDVTOA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEEP-VEIN THROMBOSIS; TUMOR ANGIOGENESIS; INTERLEUKIN-8; STREPTOKINASE; INFLAMMATION; MACROPHAGE; ADHESION; HEPARIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Wakefield, TW Univ Michigan, Med Ctr, Dept Surg,Jobst Vasc Surg Lab, Vasc Surg Sect,Taubman Hlth Care Ctr 2210, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA Univ Michigan 1500 E Med Ctr Dr Ann Arbor MI USA 48109 9 USA
Citazione:
T.W. Wakefield et al., "Neovascularization during venous thrombosis organization: A preliminary study", J VASC SURG, 30(5), 1999, pp. 885-892

Abstract

Purpose: Thrombus organization after venous thromboembolism leading to recanalization occurs at a variable rate. The angiogenic chemokine interleukin-8 (IL-8) has been found in thrombus months after thrombus initiation. We hypothesize that thrombus organization involves neovascularization and leukocyte influx and that IL-8 administered at thrombus induction will promote thrombus organization. Methods: A group of rats underwent inferior vena caval occlusive thrombosis. At thrombus induction and every 24 hours, the rats were administered IL-8 (1 mu g) or serum albumin. The rats were killed at either day 4, day 8, or day 12, and, at death, colloidal carbon was perfused via the heart. The inferior vena cava was isolated, measured, weighed, and formalin fixed. The sections were stained with anti-polymorphonuclear leukocyte antibody, the endothelial marker factor VIII-related antigen, and with hematoxylin and eosin. Thrombus neovascularization (colloidal carbon) with morphometric analysis was normalized to the total thrombus area. In addition, the rats underwent perfusion with fluorescein isothiocyanate dextran (molecular weight, 150,000) at death to correlate with colloidal carbon perfusion, and thrombus fluorescence was determined. Results: Thrombus cellularity initially involved neutrophils, followed by monocytes. Significantly more neutrophils, monocytes, and cells that were defined as spindle shaped (fibroblasts and endothelial cells) were noted in the animals treated with IL-8. Neovascularization was significantly increased at day 4 in the animals treated with IL-8 versus the animals treated with Serum albumin and was corroborated with a significant increase in thrombus fluorescein isothiocyanate dextran fluorescence at day 4 in the rats treated with IL-8. Colloidal carbon perfusion was noted within vascular channels without extravasation and colocalized with factor VIII-related antigen. Conclusion: This study shows that thrombus organization involves neovascularization and that IL-8 augments thrombus organization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 07:56:37